A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication

A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication

A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective...

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Main Author: C. Abolnik
Format: Article
Language:English
Published: AOSIS 2008-09-01
Series:Onderstepoort Journal of Veterinary Research
Online Access:https://ojvr.org/index.php/ojvr/article/view/110
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spelling doaj-6315fbc2eb8f44bd8b7e1fa0a42905a42020-11-24T22:59:52ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352008-09-0175434735110.4102/ojvr.v75i4.110102A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communicationC. AbolnikA Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.https://ojvr.org/index.php/ojvr/article/view/110
collection DOAJ
language English
format Article
sources DOAJ
author C. Abolnik
spellingShingle C. Abolnik
A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
Onderstepoort Journal of Veterinary Research
author_facet C. Abolnik
author_sort C. Abolnik
title A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
title_short A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
title_full A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
title_fullStr A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
title_full_unstemmed A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
title_sort rapid and sensitive real-time reverse transcription pcr for the pathotyping of south african h5n2 avian influenza viruses : research communication
publisher AOSIS
series Onderstepoort Journal of Veterinary Research
issn 0030-2465
2219-0635
publishDate 2008-09-01
description A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.
url https://ojvr.org/index.php/ojvr/article/view/110
work_keys_str_mv AT cabolnik arapidandsensitiverealtimereversetranscriptionpcrforthepathotypingofsouthafricanh5n2avianinfluenzavirusesresearchcommunication
AT cabolnik rapidandsensitiverealtimereversetranscriptionpcrforthepathotypingofsouthafricanh5n2avianinfluenzavirusesresearchcommunication
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