Detection of pathogenic microorganisms in urine samples with BluBAC system

• Main Authors: Nardo Ramírez-Frómeta, Carlos Abel Lamothe-Nuviola, Elier Riverón-Rodríguez, Carmen Yamilet Moreno-Barrios, Angel Regueiro-Gómez
• Format: Article
• Language: English
• Published: Universidad Católica de Oriente 2018-02-01
• Series: Bionatura
• Subjects: Microbiological diagnostics; urinary tract infections; enterobacteria; blue light; photostimulation; BluBAC.
• Online Access: http://revistabionatura.com/2018.03.01.5.html
• ISSN: 1390-9347; 1390-9355

The main purpose of this research was to evaluate the performance of BluBAC- an innovative system for the detection of pathogenic microorganisms in urine samples (uroculture). BluBAC system is based on the combination of photostimulation and turbidimetry. The urine samples were analyzed by the following three methods: direct count in Petri dish (reference method), DIRAMIC system and BluBAC system. Based on the conventional protocol for the obtention of samples for the analysis of uroculture, 350 samples from inpatients and outpatients. Results show a 100 % coincidence with the reference method and a detection time lower than 20 minutes in the 90 samples classified as positive. Although the obtained results must be amplified and corroborated with other studies, the sensitivity shown by BluBAC system in the detection of various enterobacteria is a new alternative to the diagnosis of urinary tract infections if consideration is given to the high costs of the systems for the rapid microbiological diagnosis available in the market and their negative impact on the economy of small and medium-sized laboratories, clinics and hospitals.