Survival of isolated skin explants in remote interaction with stratiform periodic structures
Full-thickness skin grafts are used in reconstructive surgeries. Objectives Experimental study of the possibility of long-term preservation of viable skin grafts in severely impaired trophics at remote interaction with entities having stratiform periodic structure. Material and methods Full-thickne...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics
2021-04-01
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| Series: | Гений oртопедии |
| Subjects: |
| Summary: | Full-thickness skin grafts are used in reconstructive surgeries. Objectives Experimental study of the possibility of long-term preservation of viable
skin grafts in severely impaired trophics at remote interaction with entities having stratiform periodic structure. Material and methods Full-thickness
skin was excised from the rabbits' backside and dissected into explants sized 1.0 × 1.0 cm. The samples (n = 81) were divided into three groups and
thermostated for 2 days at 37 °C at a various distance from the metal (aluminum) presented as a 20-layer package of smooth foil forming a stratiform
periodic structure (SPS) (series I), chaotic layers of squeezed foil (series II) and a single-piece sheet (series III). Histological analysis was performed
for the three series to evaluate the explants' viability after the thermostating. Results The highest survival estimates were seen in experimental explants
of series I that interacted with the SPS of stratified foil layers. The wave nature of such remote interaction was suggested with delayed dystrophic and
necrotic processes developing in the skin samples. Experimental samples of series III appeared to be less viable. The explant vitality in series II was
sharply reduced due to rapidly spreading necrosis. Conclusion Skin explants were shown to retain viability for a longer time when interacting remotely
with stratiform periodic structures in the absent trophics. These promising results can be practical for the development of wound dressings to improve
survival of full-thickness skin transplantation in reconstruction of deep skin defects. |
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| ISSN: | 1028-4427 2542-131X |