Expression of Heat Shock Protein 70 Is Insufficient To Extend Drosophila melanogaster Longevity

• Main Authors: Chengfeng Xiao, Danna Hull, Shuang Qiu, Joanna Yeung, Jie Zheng, Taylor Barwell, R. Meldrum Robertson, Laurent Seroude
• Format: Article
• Language: English
• Published: Oxford University Press 2019-12-01
• Series: G3: Genes, Genomes, Genetics
• Subjects: uas/gal4; muscle; nervous system; stress resistance; development
• Online Access: http://g3journal.org/lookup/doi/10.1534/g3.119.400782

It has been known for over 20 years that Drosophila melanogaster flies with twelve additional copies of the hsp70 gene encoding the 70 kD heat shock protein lives longer after a non-lethal heat treatment. Since the heat treatment also induces the expression of additional heat shock proteins, the biological effect can be due either to HSP70 acting alone or in combination. This study used the UAS/GAL4 system to determine whether hsp70 is sufficient to affect the longevity and the resistance to thermal, oxidative or desiccation stresses of the whole organism. We observed that HSP70 expression in the nervous system or muscles has no effect on longevity or stress resistance but ubiquitous expression reduces the life span of males. We also observed that the down-regulation of hsp70 using RNAi did not affect longevity.