Specific methylation of plasma nonesterified fatty acids in a one-step reaction.
Analysis of plasma nonesterified fatty acids (NEFA) by gas-liquid chromatography requires procedures that are both lengthy and cumbersome. A 45-min direct methylation procedure was carried out at 24-29 degrees C on 150 microliter of plasma added with an internal standard in 5.0 ml of methanol-acetyl...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
1988-02-01
|
Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520385539 |