Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

• Main Authors: Lísia Borges Attílio, Francisco de Assis Alves Mourão Filho, Ricardo Harakava, Tatiane Loureiro da Silva, Luzia Yuriko Miyata, Liliane Cristina Libório Stipp, Beatriz Madalena Januzzi Mendes
• Format: Article
• Language: English
• Published: Embrapa Informação Tecnológica 2013-07-01
• Series: Pesquisa Agropecuária Brasileira
• Subjects: Citrus sinensis; peptídeo antimicrobiano; promotor tecido‑específico
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000700006&lng=en&tlng=en
• ISSN: 1678-3921

The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.