Shape selection and mis-assembly in viral capsid formation by elastic frustration

• Main Authors: Carlos I Mendoza, David Reguera
• Format: Article
• Language: English
• Published: eLife Sciences Publications Ltd 2020-04-01
• Series: eLife
• Subjects: virus; self-assembly; continuum elasticity theory; brownian dynamics simulations
• Online Access: https://elifesciences.org/articles/52525

The successful assembly of a closed protein shell (or capsid) is a key step in the replication of viruses and in the production of artificial viral cages for bio/nanotechnological applications. During self-assembly, the favorable binding energy competes with the energetic cost of the growing edge and the elastic stresses generated due to the curvature of the capsid. As a result, incomplete structures such as open caps, cylindrical or ribbon-shaped shells may emerge, preventing the successful replication of viruses. Using elasticity theory and coarse-grained simulations, we analyze the conditions required for these processes to occur and their significance for empty virus self-assembly. We find that the outcome of the assembly can be recast into a universal phase diagram showing that viruses with high mechanical resistance cannot be self-assembled directly as spherical structures. The results of our study justify the need of a maturation step and suggest promising routes to hinder viral infections by inducing mis-assembly.