In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps

Per Sandgren,1,2 Veronica Berggren,3 Carl Westling,1,2 Viveka Stiller1 1Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, 2Department of Laboratory Medicine, Karolinska Institutet, 3Department of Neonatology, Karolinska University Hospital, Stockholm, Sweden...

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Main Authors: Sandgren P, Berggren V, Westling C, Stiller V
Format: Article
Language:English
Published: Dove Medical Press 2014-12-01
Series:International Journal of Clinical Transfusion Medicine
Online Access:http://www.dovepress.com/in-vitro-viability-effects-on-apheresis-and-buffy-coat-derived-platele-peer-reviewed-article-IJCTM
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spelling doaj-61761b6b6fc94f6e933c2bb048c2a17c2020-11-24T23:16:50ZengDove Medical PressInternational Journal of Clinical Transfusion Medicine2253-32492014-12-012014default738019387In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumpsSandgren PBerggren VWestling CStiller VPer Sandgren,1,2 Veronica Berggren,3 Carl Westling,1,2 Viveka Stiller1 1Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, 2Department of Laboratory Medicine, Karolinska Institutet, 3Department of Neonatology, Karolinska University Hospital, Stockholm, SwedenBackground: Different infusion pump systems as well as gravity infusion have been widely used in neonatal transfusion. However, the limited number of published studies describing the use of infusion pumps on platelets illustrates the necessity for more robust data.Methods: To evaluate the potential in vitro effects on the cellular, metabolic, functional and phenotypic properties of platelets, we set up a four-arm paired study simultaneously comparing the use of different infusion pumps (Alaris® CC/GP) with unexposed platelets. The platelet units (n=8) were either produced by the apheresis technique and suspended in 100% plasma or derived from buffy coats to yield platelet units stored in approximately 30% plasma and 70% SSP+. Fresh and 5-day old platelets were tested.Results: Regardless of the production system or storage time used, no significant differences were observed in glucose and lactate concentration, pH, adenosine triphosphate levels, response to extent of shape change, hypotonic shock response reactivity, and CD62P expression. Similarly, no differences were observed in expression of the conformational epitope on glycoprotein IIb/IIIa, determined using procaspase-activating compound 1, or in the expression of CD42b and platelet-endothelial cell adhesion molecule-1 in a comparison between platelets administered through infusion pumps versus unexposed platelets.Conclusion: Using Alaris CC/GP infusion pumps had no influence on the cellular, functional, and phenotypic in vitro properties of platelets. This fact seems not to be affected by different production systems or storage time.Keywords: platelets, neonatal platelet transfusionhttp://www.dovepress.com/in-vitro-viability-effects-on-apheresis-and-buffy-coat-derived-platele-peer-reviewed-article-IJCTM
collection DOAJ
language English
format Article
sources DOAJ
author Sandgren P
Berggren V
Westling C
Stiller V
spellingShingle Sandgren P
Berggren V
Westling C
Stiller V
In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
International Journal of Clinical Transfusion Medicine
author_facet Sandgren P
Berggren V
Westling C
Stiller V
author_sort Sandgren P
title In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
title_short In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
title_full In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
title_fullStr In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
title_full_unstemmed In vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
title_sort in vitro viability effects on apheresis and buffy-coat derived platelets administered through infusion pumps
publisher Dove Medical Press
series International Journal of Clinical Transfusion Medicine
issn 2253-3249
publishDate 2014-12-01
description Per Sandgren,1,2 Veronica Berggren,3 Carl Westling,1,2 Viveka Stiller1 1Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, 2Department of Laboratory Medicine, Karolinska Institutet, 3Department of Neonatology, Karolinska University Hospital, Stockholm, SwedenBackground: Different infusion pump systems as well as gravity infusion have been widely used in neonatal transfusion. However, the limited number of published studies describing the use of infusion pumps on platelets illustrates the necessity for more robust data.Methods: To evaluate the potential in vitro effects on the cellular, metabolic, functional and phenotypic properties of platelets, we set up a four-arm paired study simultaneously comparing the use of different infusion pumps (Alaris® CC/GP) with unexposed platelets. The platelet units (n=8) were either produced by the apheresis technique and suspended in 100% plasma or derived from buffy coats to yield platelet units stored in approximately 30% plasma and 70% SSP+. Fresh and 5-day old platelets were tested.Results: Regardless of the production system or storage time used, no significant differences were observed in glucose and lactate concentration, pH, adenosine triphosphate levels, response to extent of shape change, hypotonic shock response reactivity, and CD62P expression. Similarly, no differences were observed in expression of the conformational epitope on glycoprotein IIb/IIIa, determined using procaspase-activating compound 1, or in the expression of CD42b and platelet-endothelial cell adhesion molecule-1 in a comparison between platelets administered through infusion pumps versus unexposed platelets.Conclusion: Using Alaris CC/GP infusion pumps had no influence on the cellular, functional, and phenotypic in vitro properties of platelets. This fact seems not to be affected by different production systems or storage time.Keywords: platelets, neonatal platelet transfusion
url http://www.dovepress.com/in-vitro-viability-effects-on-apheresis-and-buffy-coat-derived-platele-peer-reviewed-article-IJCTM
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