Germline Stem Cells Drive Ovary Regeneration in Zebrafish

Summary: Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that...

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Main Authors: Zigang Cao, Xiaoyu Mao, Lingfei Luo
Format: Article
Language:English
Published: Elsevier 2019-02-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124719300907
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spelling doaj-6149fac75d164f03b6f0556af06e58cc2020-11-25T01:11:13ZengElsevierCell Reports2211-12472019-02-0126717091717.e3Germline Stem Cells Drive Ovary Regeneration in ZebrafishZigang Cao0Xiaoyu Mao1Lingfei Luo2Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, China; Jiangxi Key Laboratory of Organ Developmental Biology, College of Life Sciences, JingGangShan University, Ji’an, 343009 Jiangxi, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, China; Corresponding authorSummary: Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration. : Germline stem cells maintain ovarian high fecundity throughout the life cycle in zebrafish. Cao et al. demonstrate that the GSC marker nanos2 is required for the maintenance of GSCs and provide evidence that ovary regeneration is a GSC-driven process in zebrafish. Keywords: GSCs, nanos2, zebrafish, ovary regeneration, Wnthttp://www.sciencedirect.com/science/article/pii/S2211124719300907
collection DOAJ
language English
format Article
sources DOAJ
author Zigang Cao
Xiaoyu Mao
Lingfei Luo
spellingShingle Zigang Cao
Xiaoyu Mao
Lingfei Luo
Germline Stem Cells Drive Ovary Regeneration in Zebrafish
Cell Reports
author_facet Zigang Cao
Xiaoyu Mao
Lingfei Luo
author_sort Zigang Cao
title Germline Stem Cells Drive Ovary Regeneration in Zebrafish
title_short Germline Stem Cells Drive Ovary Regeneration in Zebrafish
title_full Germline Stem Cells Drive Ovary Regeneration in Zebrafish
title_fullStr Germline Stem Cells Drive Ovary Regeneration in Zebrafish
title_full_unstemmed Germline Stem Cells Drive Ovary Regeneration in Zebrafish
title_sort germline stem cells drive ovary regeneration in zebrafish
publisher Elsevier
series Cell Reports
issn 2211-1247
publishDate 2019-02-01
description Summary: Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration. : Germline stem cells maintain ovarian high fecundity throughout the life cycle in zebrafish. Cao et al. demonstrate that the GSC marker nanos2 is required for the maintenance of GSCs and provide evidence that ovary regeneration is a GSC-driven process in zebrafish. Keywords: GSCs, nanos2, zebrafish, ovary regeneration, Wnt
url http://www.sciencedirect.com/science/article/pii/S2211124719300907
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AT xiaoyumao germlinestemcellsdriveovaryregenerationinzebrafish
AT lingfeiluo germlinestemcellsdriveovaryregenerationinzebrafish
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