Germline Stem Cells Drive Ovary Regeneration in Zebrafish
Summary: Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that...
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doaj-6149fac75d164f03b6f0556af06e58cc2020-11-25T01:11:13ZengElsevierCell Reports2211-12472019-02-0126717091717.e3Germline Stem Cells Drive Ovary Regeneration in ZebrafishZigang Cao0Xiaoyu Mao1Lingfei Luo2Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, China; Jiangxi Key Laboratory of Organ Developmental Biology, College of Life Sciences, JingGangShan University, Ji’an, 343009 Jiangxi, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, China; Corresponding authorSummary: Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration. : Germline stem cells maintain ovarian high fecundity throughout the life cycle in zebrafish. Cao et al. demonstrate that the GSC marker nanos2 is required for the maintenance of GSCs and provide evidence that ovary regeneration is a GSC-driven process in zebrafish. Keywords: GSCs, nanos2, zebrafish, ovary regeneration, Wnthttp://www.sciencedirect.com/science/article/pii/S2211124719300907 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zigang Cao Xiaoyu Mao Lingfei Luo |
spellingShingle |
Zigang Cao Xiaoyu Mao Lingfei Luo Germline Stem Cells Drive Ovary Regeneration in Zebrafish Cell Reports |
author_facet |
Zigang Cao Xiaoyu Mao Lingfei Luo |
author_sort |
Zigang Cao |
title |
Germline Stem Cells Drive Ovary Regeneration in Zebrafish |
title_short |
Germline Stem Cells Drive Ovary Regeneration in Zebrafish |
title_full |
Germline Stem Cells Drive Ovary Regeneration in Zebrafish |
title_fullStr |
Germline Stem Cells Drive Ovary Regeneration in Zebrafish |
title_full_unstemmed |
Germline Stem Cells Drive Ovary Regeneration in Zebrafish |
title_sort |
germline stem cells drive ovary regeneration in zebrafish |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2019-02-01 |
description |
Summary: Germline stem cells (GSCs) sustain gametogenesis during the organismal life cycle. Although evidence suggests that GSCs are consistently present in the zebrafish ovary and support oogenesis, whether GSCs are involved in zebrafish ovary regeneration is poorly understood. Here, we found that nanos2, a conserved vertebrate GSC marker, is required for maintaining GSCs in zebrafish. We applied genetic ablation and tissue resection techniques to delineate the function of GSCs in zebrafish ovary regeneration. After GSC ablation, ovaries fail to regenerate and are converted to sterile testes. Amputated ovarian tissues completely regenerate as a result of the proliferation of residual GSCs, but nanos2 mutant ovaries fail to regenerate after amputation due to a lack of GSCs. The repression of Wnt signaling leads to reduced numbers of GSCs and delayed ovary regeneration. Our results provide insight into the key role of GSCs in driving ovary regeneration. : Germline stem cells maintain ovarian high fecundity throughout the life cycle in zebrafish. Cao et al. demonstrate that the GSC marker nanos2 is required for the maintenance of GSCs and provide evidence that ovary regeneration is a GSC-driven process in zebrafish. Keywords: GSCs, nanos2, zebrafish, ovary regeneration, Wnt |
url |
http://www.sciencedirect.com/science/article/pii/S2211124719300907 |
work_keys_str_mv |
AT zigangcao germlinestemcellsdriveovaryregenerationinzebrafish AT xiaoyumao germlinestemcellsdriveovaryregenerationinzebrafish AT lingfeiluo germlinestemcellsdriveovaryregenerationinzebrafish |
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