Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility

• Main Authors: José Ronaldo Vasconcelos Graça, Mirna Marques Bezerra, Vilma Lima, José Ariévilo Gurgel Rodrigues, Diego Levi Silveira Monteiro, Ana Luíza Gomes Quinderé, Rodrigo César das Neves Amorim, Regina Célia Monteiro de Paula, Norma Maria Barros Benevides
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2011-10-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: Halymenia floresia; sulfated polysaccharides; gut motility
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500008
• ISSN: 1516-8913; 1678-4324

The aim of this work was to study the effect of Halymenia floresia (Hf) on duodenum contractility, and on experimental protocols of gastric compliance (GC) in rats. Fraction Hf2s exhibited a concentration-dependent myocontractile effect (EC50 12.48 µg/ml), and an inhibitory effect after consecutive washing. The contractile response promoted by Hf2s in the duodenum strips was completely inhibited by verapamil, and the effects were prevented in the presence of Ca2+-free medium. The pretreatment with atropine prevented the Hf2s myocontractile effect. Hf2s was also capable to decrease the GC (from 3.8±0.06 to 3.4±0.13 ml, P<0.05), which did not return to basal levels after more 50 min of observation. These results indicated that the algal polysaccharide possessed in vitro and in vivo gastrointestinal effects.