Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes

Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlan...

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Main Authors: Youngjin Park, Qirui Zhang, Geert F. Wiegertjes, Jorge M.O. Fernandes, Viswanath Kiron
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fcell.2020.580848/full
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spelling doaj-613f07427a22455c92873036369ced6c2020-11-25T03:43:35ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-10-01810.3389/fcell.2020.580848580848Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific GenesYoungjin Park0Qirui Zhang1Geert F. Wiegertjes2Jorge M.O. Fernandes3Viswanath Kiron4Faculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayDivision of Clinical Genetics, Lund University, Lund, SwedenAquaculture and Fisheries Group, Wageningen University & Research, Wageningen, NetherlandsFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayOur knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.https://www.frontiersin.org/article/10.3389/fcell.2020.580848/fulladherentintestinal cellsImageStream®XAtlantic salmonphagocytosisRNA-Seq
collection DOAJ
language English
format Article
sources DOAJ
author Youngjin Park
Qirui Zhang
Geert F. Wiegertjes
Jorge M.O. Fernandes
Viswanath Kiron
spellingShingle Youngjin Park
Qirui Zhang
Geert F. Wiegertjes
Jorge M.O. Fernandes
Viswanath Kiron
Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
Frontiers in Cell and Developmental Biology
adherent
intestinal cells
ImageStream®X
Atlantic salmon
phagocytosis
RNA-Seq
author_facet Youngjin Park
Qirui Zhang
Geert F. Wiegertjes
Jorge M.O. Fernandes
Viswanath Kiron
author_sort Youngjin Park
title Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
title_short Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
title_full Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
title_fullStr Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
title_full_unstemmed Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
title_sort adherent intestinal cells from atlantic salmon show phagocytic ability and express macrophage-specific genes
publisher Frontiers Media S.A.
series Frontiers in Cell and Developmental Biology
issn 2296-634X
publishDate 2020-10-01
description Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.
topic adherent
intestinal cells
ImageStream®X
Atlantic salmon
phagocytosis
RNA-Seq
url https://www.frontiersin.org/article/10.3389/fcell.2020.580848/full
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AT geertfwiegertjes adherentintestinalcellsfromatlanticsalmonshowphagocyticabilityandexpressmacrophagespecificgenes
AT jorgemofernandes adherentintestinalcellsfromatlanticsalmonshowphagocyticabilityandexpressmacrophagespecificgenes
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