Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes
Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlan...
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2020-10-01
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doaj-613f07427a22455c92873036369ced6c2020-11-25T03:43:35ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-10-01810.3389/fcell.2020.580848580848Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific GenesYoungjin Park0Qirui Zhang1Geert F. Wiegertjes2Jorge M.O. Fernandes3Viswanath Kiron4Faculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayDivision of Clinical Genetics, Lund University, Lund, SwedenAquaculture and Fisheries Group, Wageningen University & Research, Wageningen, NetherlandsFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayOur knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.https://www.frontiersin.org/article/10.3389/fcell.2020.580848/fulladherentintestinal cellsImageStream®XAtlantic salmonphagocytosisRNA-Seq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Youngjin Park Qirui Zhang Geert F. Wiegertjes Jorge M.O. Fernandes Viswanath Kiron |
spellingShingle |
Youngjin Park Qirui Zhang Geert F. Wiegertjes Jorge M.O. Fernandes Viswanath Kiron Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes Frontiers in Cell and Developmental Biology adherent intestinal cells ImageStream®X Atlantic salmon phagocytosis RNA-Seq |
author_facet |
Youngjin Park Qirui Zhang Geert F. Wiegertjes Jorge M.O. Fernandes Viswanath Kiron |
author_sort |
Youngjin Park |
title |
Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes |
title_short |
Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes |
title_full |
Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes |
title_fullStr |
Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes |
title_full_unstemmed |
Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes |
title_sort |
adherent intestinal cells from atlantic salmon show phagocytic ability and express macrophage-specific genes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-10-01 |
description |
Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells. |
topic |
adherent intestinal cells ImageStream®X Atlantic salmon phagocytosis RNA-Seq |
url |
https://www.frontiersin.org/article/10.3389/fcell.2020.580848/full |
work_keys_str_mv |
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