Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with...

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Main Authors: Guangbing Wei, Xin Chen, Guanghui Wang, Lin Fan, Kang Wang, Xuqi Li
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2016-06-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:http://www.karger.com/Article/FullText/445603
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spelling doaj-613cd039e5e64ef79525f481496169172020-11-25T02:40:09ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-06-01391334610.1159/000445603445603Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat ModelGuangbing WeiXin ChenGuanghui WangLin FanKang WangXuqi LiBackground: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.http://www.karger.com/Article/FullText/445603Postoperative adhesionsResveratrolTGF-β1IL-6Inflammatory Response
collection DOAJ
language English
format Article
sources DOAJ
author Guangbing Wei
Xin Chen
Guanghui Wang
Lin Fan
Kang Wang
Xuqi Li
spellingShingle Guangbing Wei
Xin Chen
Guanghui Wang
Lin Fan
Kang Wang
Xuqi Li
Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
Cellular Physiology and Biochemistry
Postoperative adhesions
Resveratrol
TGF-β1
IL-6
Inflammatory Response
author_facet Guangbing Wei
Xin Chen
Guanghui Wang
Lin Fan
Kang Wang
Xuqi Li
author_sort Guangbing Wei
title Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
title_short Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
title_full Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
title_fullStr Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
title_full_unstemmed Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model
title_sort effect of resveratrol on the prevention of intra-abdominal adhesion formation in a rat model
publisher Cell Physiol Biochem Press GmbH & Co KG
series Cellular Physiology and Biochemistry
issn 1015-8987
1421-9778
publishDate 2016-06-01
description Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.
topic Postoperative adhesions
Resveratrol
TGF-β1
IL-6
Inflammatory Response
url http://www.karger.com/Article/FullText/445603
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