Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of...
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Journal of Pure and Applied Microbiology
2018-12-01
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doaj-6129c6c4c9a4430d88985b03ec746d212021-10-02T18:52:29ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011242159216410.22207/JPAM.12.4.54Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice GenotypesRaghavendrarao Sanagala0Ranjitha Kumari B.D.1Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu - 620 024, India.Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu - 620 024, India.A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of embryogenic calli or regeneration of fertile plants from transformed cells. In this study, standardized regeneration system for indica rice cultivars was carried out. Further the expression of miR164c was investigated in the different rice genotypes. The expression of miR164c was proportional to the percentage of regeneration of the rice genotypes. These results provide interesting insights that will significantly improve our understanding of direct regeneration of rice from callus tissue.https://microbiologyjournal.org/expression-analysis-of-mirna-164c-during-rice-regeneration-in-different-indica-rice-genotypes/mirnatissue culturerice regenerationgenotypes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raghavendrarao Sanagala Ranjitha Kumari B.D. |
spellingShingle |
Raghavendrarao Sanagala Ranjitha Kumari B.D. Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes Journal of Pure and Applied Microbiology mirna tissue culture rice regeneration genotypes |
author_facet |
Raghavendrarao Sanagala Ranjitha Kumari B.D. |
author_sort |
Raghavendrarao Sanagala |
title |
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes |
title_short |
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes |
title_full |
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes |
title_fullStr |
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes |
title_full_unstemmed |
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes |
title_sort |
expression analysis of mirna 164c during rice regeneration in different indica rice genotypes |
publisher |
Journal of Pure and Applied Microbiology |
series |
Journal of Pure and Applied Microbiology |
issn |
0973-7510 2581-690X |
publishDate |
2018-12-01 |
description |
A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for
achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic
transformation and require refinement of the tissue culture protocol for the generation of embryogenic
calli or regeneration of fertile plants from transformed cells. In this study, standardized regeneration
system for indica rice cultivars was carried out. Further the expression of miR164c was investigated
in the different rice genotypes. The expression of miR164c was proportional to the percentage of
regeneration of the rice genotypes. These results provide interesting insights that will significantly
improve our understanding of direct regeneration of rice from callus tissue. |
topic |
mirna tissue culture rice regeneration genotypes |
url |
https://microbiologyjournal.org/expression-analysis-of-mirna-164c-during-rice-regeneration-in-different-indica-rice-genotypes/ |
work_keys_str_mv |
AT raghavendraraosanagala expressionanalysisofmirna164cduringriceregenerationindifferentindicaricegenotypes AT ranjithakumaribd expressionanalysisofmirna164cduringriceregenerationindifferentindicaricegenotypes |
_version_ |
1716848650148118528 |