Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes

A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of...

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Main Authors: Raghavendrarao Sanagala, Ranjitha Kumari B.D.
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2018-12-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/expression-analysis-of-mirna-164c-during-rice-regeneration-in-different-indica-rice-genotypes/
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spelling doaj-6129c6c4c9a4430d88985b03ec746d212021-10-02T18:52:29ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011242159216410.22207/JPAM.12.4.54Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice GenotypesRaghavendrarao Sanagala0Ranjitha Kumari B.D.1Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu - 620 024, India.Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu - 620 024, India.A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of embryogenic calli or regeneration of fertile plants from transformed cells. In this study, standardized regeneration system for indica rice cultivars was carried out. Further the expression of miR164c was investigated in the different rice genotypes. The expression of miR164c was proportional to the percentage of regeneration of the rice genotypes. These results provide interesting insights that will significantly improve our understanding of direct regeneration of rice from callus tissue.https://microbiologyjournal.org/expression-analysis-of-mirna-164c-during-rice-regeneration-in-different-indica-rice-genotypes/mirnatissue culturerice regenerationgenotypes
collection DOAJ
language English
format Article
sources DOAJ
author Raghavendrarao Sanagala
Ranjitha Kumari B.D.
spellingShingle Raghavendrarao Sanagala
Ranjitha Kumari B.D.
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
Journal of Pure and Applied Microbiology
mirna
tissue culture
rice regeneration
genotypes
author_facet Raghavendrarao Sanagala
Ranjitha Kumari B.D.
author_sort Raghavendrarao Sanagala
title Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
title_short Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
title_full Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
title_fullStr Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
title_full_unstemmed Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
title_sort expression analysis of mirna 164c during rice regeneration in different indica rice genotypes
publisher Journal of Pure and Applied Microbiology
series Journal of Pure and Applied Microbiology
issn 0973-7510
2581-690X
publishDate 2018-12-01
description A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of embryogenic calli or regeneration of fertile plants from transformed cells. In this study, standardized regeneration system for indica rice cultivars was carried out. Further the expression of miR164c was investigated in the different rice genotypes. The expression of miR164c was proportional to the percentage of regeneration of the rice genotypes. These results provide interesting insights that will significantly improve our understanding of direct regeneration of rice from callus tissue.
topic mirna
tissue culture
rice regeneration
genotypes
url https://microbiologyjournal.org/expression-analysis-of-mirna-164c-during-rice-regeneration-in-different-indica-rice-genotypes/
work_keys_str_mv AT raghavendraraosanagala expressionanalysisofmirna164cduringriceregenerationindifferentindicaricegenotypes
AT ranjithakumaribd expressionanalysisofmirna164cduringriceregenerationindifferentindicaricegenotypes
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