Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/expression-analysis-of-mirna-164c-during-rice-regeneration-in-different-indica-rice-genotypes/ |
| Summary: | A highly efficient and reproducible tissue culture regeneration system is an important prerequisite for
achieving successful transformation. However, most indica rice varieties remain recalcitrant to genetic
transformation and require refinement of the tissue culture protocol for the generation of embryogenic
calli or regeneration of fertile plants from transformed cells. In this study, standardized regeneration
system for indica rice cultivars was carried out. Further the expression of miR164c was investigated
in the different rice genotypes. The expression of miR164c was proportional to the percentage of
regeneration of the rice genotypes. These results provide interesting insights that will significantly
improve our understanding of direct regeneration of rice from callus tissue. |
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| ISSN: | 0973-7510 2581-690X |