Experimental study about Hepatitis B virus X protein (HBx) promotion on the liver cancer cell line growth as well as its molecular mechanism

• Main Authors: Li Fan, Jian-Ting Chen
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-07-01
• Series: Journal of Hainan Medical University
• Subjects: Primary hepatic carcinoma; Hepatitis B virus X protein; Proliferation; Invasion; Angiogenesis
• Online Access: http://www.hnykdxxb.com/PDF/201713/1.pdf

Objective: To study the effect of hepatitis B virus X protein (HBx) on the expression of proliferation molecules, invasion molecules and angiogenesis molecules in liver cancer cell lines. Methods: Liver cancer cell lines HepG2 were cultured and divided into HBx group and control group that were transfected with pcDNA3.1-HBx plasmid and blank pcDNA3.1 plasmid respectively. 24 h and 48 h after transfection, the mRNA expression of proliferation molecules Survivin, cyclinD1 and c-myc, invasion molecules CD44v6, MT1-MMP, MMP2 and MMP7 as well as angiogenesis molecules VEGF, Ang-1, Ang-2 and FGF-2 in cells were determined. Results: After 24 h and 48 h of transfection, the Survivin, cyclinD1, c-myc, CD44v6, MT1-MMP, MMP2, MMP7, VEGF, Ang-1, Ang-2 and FGF-2 mRNA expression in HBx group of cells were significantly higher than those in control group. Conclusion: HBx can promote the expression of proliferation molecules, invasion molecules and angiogenesis molecules in the liver cancer cell lines.