Experimental study about Hepatitis B virus X protein (HBx) promotion on the liver cancer cell line growth as well as its molecular mechanism
Objective: To study the effect of hepatitis B virus X protein (HBx) on the expression of proliferation molecules, invasion molecules and angiogenesis molecules in liver cancer cell lines. Methods: Liver cancer cell lines HepG2 were cultured and divided into HBx group and control group that were t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2017-07-01
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| Series: | Journal of Hainan Medical University |
| Subjects: | |
| Online Access: | http://www.hnykdxxb.com/PDF/201713/1.pdf |
| Summary: | Objective: To study the effect of hepatitis B virus X protein (HBx) on the expression of
proliferation molecules, invasion molecules and angiogenesis molecules in liver cancer cell
lines. Methods: Liver cancer cell lines HepG2 were cultured and divided into HBx group
and control group that were transfected with pcDNA3.1-HBx plasmid and blank pcDNA3.1
plasmid respectively. 24 h and 48 h after transfection, the mRNA expression of proliferation
molecules Survivin, cyclinD1 and c-myc, invasion molecules CD44v6, MT1-MMP, MMP2
and MMP7 as well as angiogenesis molecules VEGF, Ang-1, Ang-2 and FGF-2 in cells were
determined. Results: After 24 h and 48 h of transfection, the Survivin, cyclinD1, c-myc,
CD44v6, MT1-MMP, MMP2, MMP7, VEGF, Ang-1, Ang-2 and FGF-2 mRNA expression in
HBx group of cells were significantly higher than those in control group. Conclusion: HBx
can promote the expression of proliferation molecules, invasion molecules and angiogenesis
molecules in the liver cancer cell lines. |
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| ISSN: | 1007-1237 1007-1237 |