Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study

Rosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The cont...

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Main Authors: Elizabeta Lohova, Mara Pilmane, Mara Rone-Kupfere, Janis Kisis
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Cosmetics
Subjects:
Online Access:https://www.mdpi.com/2079-9284/7/4/82
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spelling doaj-60e696c6814f458bb5e690890c7552152020-11-25T04:03:25ZengMDPI AGCosmetics2079-92842020-10-017828210.3390/cosmetics7040082Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot studyElizabeta Lohova0Mara Pilmane1Mara Rone-Kupfere2Janis Kisis3Institute of Anatomy and Anthropology, Riga Stradins University, Kronvalda Boulevard 9, LV-1010 Riga, LatviaInstitute of Anatomy and Anthropology, Riga Stradins University, Kronvalda Boulevard 9, LV-1010 Riga, LatviaClinic of Aesthetic Dermatology, Firsa Sadovnikova str. 20, LV-1003 Riga, LatviaClinic of Aesthetic Dermatology, Firsa Sadovnikova str. 20, LV-1003 Riga, LatviaRosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The control group (CG) included four facial skin samples (49 to 70 years). Routine staining and immunohistochemistry for IL-1, IL-10, LL-37, HBD-2, and HBD-4 proceeded. Results: Inflammation was observed in all the rosacea samples. A statistically significant difference was seen between epithelial HBD-2 positive cells in comparison to the control. There was a strong positive correlation between HBD-4 in the epithelium and HBD-4 in the connective tissue, IL-10 in the epithelium and IL-1 in the connective tissue, and IL-1 in the epithelium and IL-10 in the connective tissue. Conclusion: Increased levels of IL-10 and decreased levels of IL-1 show the balance between anti- and pro-inflammatory tissue responses. A significant amount of HBD-2 in the epithelium proves its important role in the local immune response of rosacea-affected tissue. The last effect seems to be intensified by the elevated level of LL-37 in the epithelium.https://www.mdpi.com/2079-9284/7/4/82inflammatory mediatorsfacial skinrosaceaimmune response
collection DOAJ
language English
format Article
sources DOAJ
author Elizabeta Lohova
Mara Pilmane
Mara Rone-Kupfere
Janis Kisis
spellingShingle Elizabeta Lohova
Mara Pilmane
Mara Rone-Kupfere
Janis Kisis
Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
Cosmetics
inflammatory mediators
facial skin
rosacea
immune response
author_facet Elizabeta Lohova
Mara Pilmane
Mara Rone-Kupfere
Janis Kisis
author_sort Elizabeta Lohova
title Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
title_short Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
title_full Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
title_fullStr Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
title_full_unstemmed Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
title_sort characterization of pro- and anti-inflammatory tissue factors in rosacea: a pilot study
publisher MDPI AG
series Cosmetics
issn 2079-9284
publishDate 2020-10-01
description Rosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The control group (CG) included four facial skin samples (49 to 70 years). Routine staining and immunohistochemistry for IL-1, IL-10, LL-37, HBD-2, and HBD-4 proceeded. Results: Inflammation was observed in all the rosacea samples. A statistically significant difference was seen between epithelial HBD-2 positive cells in comparison to the control. There was a strong positive correlation between HBD-4 in the epithelium and HBD-4 in the connective tissue, IL-10 in the epithelium and IL-1 in the connective tissue, and IL-1 in the epithelium and IL-10 in the connective tissue. Conclusion: Increased levels of IL-10 and decreased levels of IL-1 show the balance between anti- and pro-inflammatory tissue responses. A significant amount of HBD-2 in the epithelium proves its important role in the local immune response of rosacea-affected tissue. The last effect seems to be intensified by the elevated level of LL-37 in the epithelium.
topic inflammatory mediators
facial skin
rosacea
immune response
url https://www.mdpi.com/2079-9284/7/4/82
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AT marapilmane characterizationofproandantiinflammatorytissuefactorsinrosaceaapilotstudy
AT mararonekupfere characterizationofproandantiinflammatorytissuefactorsinrosaceaapilotstudy
AT janiskisis characterizationofproandantiinflammatorytissuefactorsinrosaceaapilotstudy
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