Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study
Rosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The cont...
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doaj-60e696c6814f458bb5e690890c7552152020-11-25T04:03:25ZengMDPI AGCosmetics2079-92842020-10-017828210.3390/cosmetics7040082Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot studyElizabeta Lohova0Mara Pilmane1Mara Rone-Kupfere2Janis Kisis3Institute of Anatomy and Anthropology, Riga Stradins University, Kronvalda Boulevard 9, LV-1010 Riga, LatviaInstitute of Anatomy and Anthropology, Riga Stradins University, Kronvalda Boulevard 9, LV-1010 Riga, LatviaClinic of Aesthetic Dermatology, Firsa Sadovnikova str. 20, LV-1003 Riga, LatviaClinic of Aesthetic Dermatology, Firsa Sadovnikova str. 20, LV-1003 Riga, LatviaRosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The control group (CG) included four facial skin samples (49 to 70 years). Routine staining and immunohistochemistry for IL-1, IL-10, LL-37, HBD-2, and HBD-4 proceeded. Results: Inflammation was observed in all the rosacea samples. A statistically significant difference was seen between epithelial HBD-2 positive cells in comparison to the control. There was a strong positive correlation between HBD-4 in the epithelium and HBD-4 in the connective tissue, IL-10 in the epithelium and IL-1 in the connective tissue, and IL-1 in the epithelium and IL-10 in the connective tissue. Conclusion: Increased levels of IL-10 and decreased levels of IL-1 show the balance between anti- and pro-inflammatory tissue responses. A significant amount of HBD-2 in the epithelium proves its important role in the local immune response of rosacea-affected tissue. The last effect seems to be intensified by the elevated level of LL-37 in the epithelium.https://www.mdpi.com/2079-9284/7/4/82inflammatory mediatorsfacial skinrosaceaimmune response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elizabeta Lohova Mara Pilmane Mara Rone-Kupfere Janis Kisis |
spellingShingle |
Elizabeta Lohova Mara Pilmane Mara Rone-Kupfere Janis Kisis Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study Cosmetics inflammatory mediators facial skin rosacea immune response |
author_facet |
Elizabeta Lohova Mara Pilmane Mara Rone-Kupfere Janis Kisis |
author_sort |
Elizabeta Lohova |
title |
Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study |
title_short |
Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study |
title_full |
Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study |
title_fullStr |
Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study |
title_full_unstemmed |
Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea: A Pilot study |
title_sort |
characterization of pro- and anti-inflammatory tissue factors in rosacea: a pilot study |
publisher |
MDPI AG |
series |
Cosmetics |
issn |
2079-9284 |
publishDate |
2020-10-01 |
description |
Rosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The control group (CG) included four facial skin samples (49 to 70 years). Routine staining and immunohistochemistry for IL-1, IL-10, LL-37, HBD-2, and HBD-4 proceeded. Results: Inflammation was observed in all the rosacea samples. A statistically significant difference was seen between epithelial HBD-2 positive cells in comparison to the control. There was a strong positive correlation between HBD-4 in the epithelium and HBD-4 in the connective tissue, IL-10 in the epithelium and IL-1 in the connective tissue, and IL-1 in the epithelium and IL-10 in the connective tissue. Conclusion: Increased levels of IL-10 and decreased levels of IL-1 show the balance between anti- and pro-inflammatory tissue responses. A significant amount of HBD-2 in the epithelium proves its important role in the local immune response of rosacea-affected tissue. The last effect seems to be intensified by the elevated level of LL-37 in the epithelium. |
topic |
inflammatory mediators facial skin rosacea immune response |
url |
https://www.mdpi.com/2079-9284/7/4/82 |
work_keys_str_mv |
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