Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines
Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B, containing the RRWQWR motif, were designed, synthesized, purified, and characterized using RP-HPLC chromatography and MALDI-TOF mass spectrometry. The antibacterial activity of the designed peptides against E. coli (ATCC...
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MDPI AG
2017-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/10/1641 |
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doaj-60d53df092e646c0bedb0848dd561a072020-11-24T23:55:58ZengMDPI AGMolecules1420-30492017-09-012210164110.3390/molecules22101641molecules22101641Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell LinesYerly Vargas Casanova0Jorge Antonio Rodríguez Guerra1Yadi Adriana Umaña Pérez2Aura Lucía Leal Castro3Giovanni Almanzar Reina4Javier Eduardo García Castañeda5Zuly Jenny Rivera Monroy6Biotechnology Institute, Universidad Nacional de Colombia, Carrera 45 No 26-85, 11321 Bogotá, ColombiaPharmacy Department, Universidad Nacional de Colombia, Carrera 45 No 26-85, Building 450, Office 213, 11321 Bogotá, ColombiaChemistry Department, Universidad Nacional de Colombia, Carrera 45 No 26-85, Building 450, Office 213, 11321 Bogotá, ColombiaMedicine Faculty, Universidad Nacional de Colombia, Carrera 45 No 26-85, Building 450, Office 213, 11321 Bogotá, ColombiaUniversity Children’s Hospital, University of Wurzburg, 97080 Wurzburg, GermanyPharmacy Department, Universidad Nacional de Colombia, Carrera 45 No 26-85, Building 450, Office 213, 11321 Bogotá, ColombiaChemistry Department, Universidad Nacional de Colombia, Carrera 45 No 26-85, Building 450, Office 213, 11321 Bogotá, ColombiaLinear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B, containing the RRWQWR motif, were designed, synthesized, purified, and characterized using RP-HPLC chromatography and MALDI-TOF mass spectrometry. The antibacterial activity of the designed peptides against E. coli (ATCC 11775 and 25922) and their cytotoxic effect against MDA-MB-468 and MDA-MB-231 breast cancer cell lines were evaluated. Dimeric and tetrameric peptides showed higher antibacterial activity in both bacteria strains than linear peptides. The dimeric peptide (RRWQWR)2K-Ahx exhibited the highest antibacterial activity against the tested bacterial strains. Furthermore, the peptides with high antibacterial activity exhibited significant cytotoxic effect against the tested breast cancer cell lines. This cytotoxic effect was fast and dependent on the peptide concentration. The tetrameric molecule containing RRWQWR motif has an optimal cytotoxic effect at a concentration of 22 µM. The evaluated dimeric and tetrameric peptides could be considered as candidates for developing new therapeutic agents against breast cancer. Polyvalence of linear sequences could be considered as a novel and versatile strategy for obtaining molecules with high anticancer activity.https://www.mdpi.com/1420-3049/22/10/1641lactoferricin BE. colibreast cancercytotoxic effectantibacterial activitysynthetic peptides |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yerly Vargas Casanova Jorge Antonio Rodríguez Guerra Yadi Adriana Umaña Pérez Aura Lucía Leal Castro Giovanni Almanzar Reina Javier Eduardo García Castañeda Zuly Jenny Rivera Monroy |
| spellingShingle |
Yerly Vargas Casanova Jorge Antonio Rodríguez Guerra Yadi Adriana Umaña Pérez Aura Lucía Leal Castro Giovanni Almanzar Reina Javier Eduardo García Castañeda Zuly Jenny Rivera Monroy Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines Molecules lactoferricin B E. coli breast cancer cytotoxic effect antibacterial activity synthetic peptides |
| author_facet |
Yerly Vargas Casanova Jorge Antonio Rodríguez Guerra Yadi Adriana Umaña Pérez Aura Lucía Leal Castro Giovanni Almanzar Reina Javier Eduardo García Castañeda Zuly Jenny Rivera Monroy |
| author_sort |
Yerly Vargas Casanova |
| title |
Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines |
| title_short |
Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines |
| title_full |
Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines |
| title_fullStr |
Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines |
| title_full_unstemmed |
Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines |
| title_sort |
antibacterial synthetic peptides derived from bovine lactoferricin exhibit cytotoxic effect against mda-mb-468 and mda-mb-231 breast cancer cell lines |
| publisher |
MDPI AG |
| series |
Molecules |
| issn |
1420-3049 |
| publishDate |
2017-09-01 |
| description |
Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B, containing the RRWQWR motif, were designed, synthesized, purified, and characterized using RP-HPLC chromatography and MALDI-TOF mass spectrometry. The antibacterial activity of the designed peptides against E. coli (ATCC 11775 and 25922) and their cytotoxic effect against MDA-MB-468 and MDA-MB-231 breast cancer cell lines were evaluated. Dimeric and tetrameric peptides showed higher antibacterial activity in both bacteria strains than linear peptides. The dimeric peptide (RRWQWR)2K-Ahx exhibited the highest antibacterial activity against the tested bacterial strains. Furthermore, the peptides with high antibacterial activity exhibited significant cytotoxic effect against the tested breast cancer cell lines. This cytotoxic effect was fast and dependent on the peptide concentration. The tetrameric molecule containing RRWQWR motif has an optimal cytotoxic effect at a concentration of 22 µM. The evaluated dimeric and tetrameric peptides could be considered as candidates for developing new therapeutic agents against breast cancer. Polyvalence of linear sequences could be considered as a novel and versatile strategy for obtaining molecules with high anticancer activity. |
| topic |
lactoferricin B E. coli breast cancer cytotoxic effect antibacterial activity synthetic peptides |
| url |
https://www.mdpi.com/1420-3049/22/10/1641 |
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