Thrombolytic and cytotoxic activity of different bioactive extracts of E. coli

• Main Authors: Munaza Maqsood, Zahid Mushtaq, Tahir Rasheed, Zaka Un Nisa, Farooq Sher
• Format: Article
• Language: English
• Published: Elsevier 2021-06-01
• Series: Case Studies in Chemical and Environmental Engineering
• Subjects: Escherchia coli; Cytotoxic activity; Thrombolytic activity; Clot lysis; Methanol extract and eco-friendly thrombolytic agent
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666016421000025

Novel therapeutic agents discovery have become crucial because humankind is experiencing issues regarding health and the environment. The present study is focusing on thrombolytic agents, which are therapeutics for cardiovascular diseases, eco-friendly, safe and can be used in comparison to synthetic agents that are associated with some side effects and their production is not environment friendly. The main objective of the present research was to evaluate the thrombolytic and cytotoxic potential of different extracts of Escherchia coli-ATCC35218. Soxhlet extraction method with ethanol was used for extraction from cells. Crude protein was extracted from the cell mass with protein extraction buffer. Crude isoprenoid quinone and polar lipids were extracted by using petroleum ether with methanol and chloroform as extraction solvent. In another method, the extracts were prepared using methanol as well as n-hexane, chloroform, ethyl acetate and water in serial extraction. These organic and aqueous extracts of E. coli were dried, weighed, dissolved in DMSO and tested for their hemolytic and thrombolytic potential. Mild to moderate percentage of thrombolytic activity was shown by these extracts and compared with citric acid (standard). Chloroform, ethyl acetate and methanol soluble extracts showed significant thrombolytic activities that were 43.5% ​± ​0.06, 41.8% ​± ​0.16 and 40.5% ​± ​0.07 respectively, while citric acid showed 73.7% ​± ​0.05. Maximum hemolytic activities were shown by polar lipids, crude methanol extract and n-hexane that are 87.20% ​± ​0.08, 82.5% ​± ​0.06 and 73.46%± ​0.08 respectively as compared with 0.1% Triton X-100 (positive control) with 94.75% ​± ​0.15 hemolysis. Finally, results were analyzed statistically through analysis of variance (ANOVA). Further studies on isolation and identification of bioactive thrombolytic agents from each bioactive thrombolytic extract will help us in reporting natural thrombolytic agents from E.coli that could benefit human medically. This is one of the few reports of thrombolytics available from natural sources.