Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia

• Main Authors: Zdenek Tauber, Katerina Cizkova, Maria Janikova, Jana Jurcikova, Katerina Vitkova, Lubomir Pavliska, Ludmila Porubova, Agata Krauze, Carlos Fernandez, Frantisek Jaluvka, Iveta Spackova, Ivo Lochman, Martin Prochazka, Brian H. Johnstone, Vaclav Prochazka
• Format: Article
• Language: English
• Published: Palacký University Olomouc, Faculty of Medicine and Dentistry 2019-06-01
• Series: Biomedical Papers
• Subjects: mesenchymal stem cells; tissue healing; cytokines; c-peptide; mir-126; critical limb ischemia
• Online Access: https://biomed.papers.upol.cz/artkey/bio-201902-0005_serum-c-peptide-level-correlates-with-the-course-of-muscle-tissue-healing-in-the-rabbit-model-of-critical-limb.php
• ISSN: 1213-8118; 1804-7521

Aim: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). Methods: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. Results: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. Conclusion: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.