Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

Summary: Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and...

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Bibliographic Details
Main Authors: Joe Z. Zhang, Shane Rui Zhao, Chengyi Tu, Paul Pang, Mao Zhang, Joseph C. Wu
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Microscopy
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005657
Description
Summary:Summary: Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
ISSN:2666-1667

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