Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.

Adenovirus is one of the most important causative agents of iatrogenic infections derived from contaminated medical devices or finger contact. In this study, we investigated whether nitrogen gas plasma, generated by applying a short high-voltage pulse to nitrogen using a static induction thyristor p...

Full description

Bibliographic Details
Main Authors: Akikazu Sakudo, Yoichi Toyokawa, Yuichiro Imanishi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4913946?pdf=render
id doaj-60a9cd92fc6a4f318a81bc30dce995f5
record_format Article
spelling doaj-60a9cd92fc6a4f318a81bc30dce995f52020-11-25T00:48:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01116e015792210.1371/journal.pone.0157922Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.Akikazu SakudoYoichi ToyokawaYuichiro ImanishiAdenovirus is one of the most important causative agents of iatrogenic infections derived from contaminated medical devices or finger contact. In this study, we investigated whether nitrogen gas plasma, generated by applying a short high-voltage pulse to nitrogen using a static induction thyristor power supply (1.5 kilo pulse per second), exhibited a virucidal effect against adenoviruses. Viral titer was reduced by one log within 0.94 min. Results from detection of viral capsid proteins, hexon and penton, by Western blotting and immunochromatography were unaffected by the plasma treatment. In contrast, analysis using the polymerase chain reaction suggested that plasma treatment damages the viral genomic DNA. Reactive chemical products (hydrogen peroxide, nitrate, and nitrite), ultraviolet light (UV-A) and slight temperature elevations were observed during the operation of the gas plasma device. Viral titer versus intensity of each potential virucidal factor were used to identify the primary mechanism of disinfection of adenovirus. Although exposure to equivalent levels of UV-A or heat treatment did not inactivate adenovirus, treatment with a relatively low concentration of hydrogen peroxide efficiently inactivated the virus. Our results suggest the nitrogen gas plasma generates reactive chemical products that inactivate adenovirus by damaging the viral genomic DNA.http://europepmc.org/articles/PMC4913946?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Akikazu Sakudo
Yoichi Toyokawa
Yuichiro Imanishi
spellingShingle Akikazu Sakudo
Yoichi Toyokawa
Yuichiro Imanishi
Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.
PLoS ONE
author_facet Akikazu Sakudo
Yoichi Toyokawa
Yuichiro Imanishi
author_sort Akikazu Sakudo
title Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.
title_short Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.
title_full Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.
title_fullStr Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.
title_full_unstemmed Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus.
title_sort nitrogen gas plasma generated by a static induction thyristor as a pulsed power supply inactivates adenovirus.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2016-01-01
description Adenovirus is one of the most important causative agents of iatrogenic infections derived from contaminated medical devices or finger contact. In this study, we investigated whether nitrogen gas plasma, generated by applying a short high-voltage pulse to nitrogen using a static induction thyristor power supply (1.5 kilo pulse per second), exhibited a virucidal effect against adenoviruses. Viral titer was reduced by one log within 0.94 min. Results from detection of viral capsid proteins, hexon and penton, by Western blotting and immunochromatography were unaffected by the plasma treatment. In contrast, analysis using the polymerase chain reaction suggested that plasma treatment damages the viral genomic DNA. Reactive chemical products (hydrogen peroxide, nitrate, and nitrite), ultraviolet light (UV-A) and slight temperature elevations were observed during the operation of the gas plasma device. Viral titer versus intensity of each potential virucidal factor were used to identify the primary mechanism of disinfection of adenovirus. Although exposure to equivalent levels of UV-A or heat treatment did not inactivate adenovirus, treatment with a relatively low concentration of hydrogen peroxide efficiently inactivated the virus. Our results suggest the nitrogen gas plasma generates reactive chemical products that inactivate adenovirus by damaging the viral genomic DNA.
url http://europepmc.org/articles/PMC4913946?pdf=render
work_keys_str_mv AT akikazusakudo nitrogengasplasmageneratedbyastaticinductionthyristorasapulsedpowersupplyinactivatesadenovirus
AT yoichitoyokawa nitrogengasplasmageneratedbyastaticinductionthyristorasapulsedpowersupplyinactivatesadenovirus
AT yuichiroimanishi nitrogengasplasmageneratedbyastaticinductionthyristorasapulsedpowersupplyinactivatesadenovirus
_version_ 1725256528639819776