Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)

We studied hemocytes of Biomphalaria glabrata with the use of light and transmission microscopy and flow cytometry with LysoTracker and SYTO62. These specific fluorescent dyes make it possible to distinguish cells differing in the amount of lysosomes and in transcriptional activity. We found that h...

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Bibliographic Details
Main Authors:	E E Prokhorova, M K Serebryakova, A S Tokmakova, G L Ataev
Format:	Article
Language:	English
Published:	University of Modena and Reggio Emilia 2018-09-01
Series:	Invertebrate Survival Journal
Subjects:	Biomphalaria glabrata hyalinocytes granulocytes flow cytometry LysoTracker Syto62
Online Access:	https://isj02.unimore.it/index.php/ISJ/article/view/443

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spelling	doaj-608553528750483a9c9d351f6ae5e5bd2020-12-02T18:50:10ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-09-01151Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)E E Prokhorova0M K Serebryakova1A S Tokmakova2G L Ataev3Department of Zoology, Faculty of Biology, Herzen State Pedagogical University of Russia, Russian FederationDepartment of Immunology, Institute of Experimental Medicine, Saint-Petersburg, Russian FederationDepartment of Zoology, Faculty of Biology, Herzen State Pedagogical University of Russia, Russian FederationDepartment of Zoology, Faculty of Biology, Herzen State Pedagogical University of Russia, Russian Federation We studied hemocytes of Biomphalaria glabrata with the use of light and transmission microscopy and flow cytometry with LysoTracker and SYTO62. These specific fluorescent dyes make it possible to distinguish cells differing in the amount of lysosomes and in transcriptional activity. We found that hemocytes of B. glabrata were represented by hyalinocytes and granulocytes (35.34 (27.38; 36.10) and 65.13 (64.11; 73.05) % respectively). Granulocytes were generally strongly granulated and more metabolically active than hyalinocytes. This is the first report confirming the presence of these two main cell types in the hemolymph of B. glabrata with the use of specific fluorescent dyes. https://isj02.unimore.it/index.php/ISJ/article/view/443Biomphalaria glabratahyalinocytesgranulocytesflow cytometryLysoTrackerSyto62
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	E E Prokhorova M K Serebryakova A S Tokmakova G L Ataev
spellingShingle	E E Prokhorova M K Serebryakova A S Tokmakova G L Ataev Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata) Invertebrate Survival Journal Biomphalaria glabrata hyalinocytes granulocytes flow cytometry LysoTracker Syto62
author_facet	E E Prokhorova M K Serebryakova A S Tokmakova G L Ataev
author_sort	E E Prokhorova
title	Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)
title_short	Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)
title_full	Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)
title_fullStr	Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)
title_full_unstemmed	Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)
title_sort	hemocytes of mollusc biomphalaria glabrata (gastropoda, pulmonata)
publisher	University of Modena and Reggio Emilia
series	Invertebrate Survival Journal
issn	1824-307X
publishDate	2018-09-01
description	We studied hemocytes of Biomphalaria glabrata with the use of light and transmission microscopy and flow cytometry with LysoTracker and SYTO62. These specific fluorescent dyes make it possible to distinguish cells differing in the amount of lysosomes and in transcriptional activity. We found that hemocytes of B. glabrata were represented by hyalinocytes and granulocytes (35.34 (27.38; 36.10) and 65.13 (64.11; 73.05) % respectively). Granulocytes were generally strongly granulated and more metabolically active than hyalinocytes. This is the first report confirming the presence of these two main cell types in the hemolymph of B. glabrata with the use of specific fluorescent dyes.
topic	Biomphalaria glabrata hyalinocytes granulocytes flow cytometry LysoTracker Syto62
url	https://isj02.unimore.it/index.php/ISJ/article/view/443
work_keys_str_mv	AT eeprokhorova hemocytesofmolluscbiomphalariaglabratagastropodapulmonata AT mkserebryakova hemocytesofmolluscbiomphalariaglabratagastropodapulmonata AT astokmakova hemocytesofmolluscbiomphalariaglabratagastropodapulmonata AT glataev hemocytesofmolluscbiomphalariaglabratagastropodapulmonata
_version_	1724402970989166592

Hemocytes of mollusc Biomphalaria glabrata (Gastropoda, Pulmonata)

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