Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris

Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris

Horseradish peroxidase (HRP), conjugated to antibodies and lectins, is widely used in medical diagnostics. Since recombinant production of the enzyme is difficult, HRP isolated from plant is used for these applications. Production in the yeast Pichia pastoris (P. pastoris), the most promising recomb...

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Bibliographic Details
Main Authors: Simona Capone, Lejla Ćorajević, Günther Bonifert, Patrick Murth, Daniel Maresch, Friedrich Altmann, Christoph Herwig, Oliver Spadiut
Format: Article
Language:English
Published: MDPI AG 2015-09-01
Series:International Journal of Molecular Sciences
Subjects:
horseradish peroxidase
glyco-engineering
strain engineering
bioreactor cultivation
OCH1
site-directed mutagenesis
Online Access:http://www.mdpi.com/1422-0067/16/10/23127

Internet

http://www.mdpi.com/1422-0067/16/10/23127

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