| Summary: | This work was done for optimizing the route to produce eco-friendly bacterial cellulose from bacteria in the rotten guava, and protein waste as cheap carbon/ nitrogen source in production medium. In this respect Komagataeibacter intermedius MO was isolated and identified. The protein-based waste (cheese whey) was evaluated as effective carbon/ nitrogen source in growth medium, in comparison with well-known medium (Seed medium). The role of cheese whey was studied individual and as substituting half of glucose or yeast of Seed medium. The X-ray diffraction, FT-IR spectra and TGA analysis were the techniques used in assessment together with production yield and strength properties. In comparison with seed medium the half substitution of yeast by cheese whey in seed medium exhibited enhancement of isolated Komagataeibacter intermedius MO in production of BC pellicles with high crystallinity, thermal stability and strength properties (tensile and elasticity). Moreover, despite its production yield was lower than that produced from seed medium, but it equivalent to BC production yield on using Hestrin- Schramm (HS) medium. This protein-waste suggested for modification of seed medium for economic production of BC for functional application.
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