A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.
We introduce a simple and universal cloning plasmid system for gene expression in prokaryotic (Escherichia coli) and mammalian cells. This novel system has two expression modes: the (subcloning) prokaryotic and mammalian modes. This system streamlines the process of producing mammalian gene expressi...
Main Authors: | Manabu Murakami, Takayoshi Ohba, Agnieszka M Murakami, Chong Han, Kenji Kuwasako, Shirou Itagaki |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
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Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0216169 |
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