A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.

A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.

We introduce a simple and universal cloning plasmid system for gene expression in prokaryotic (Escherichia coli) and mammalian cells. This novel system has two expression modes: the (subcloning) prokaryotic and mammalian modes. This system streamlines the process of producing mammalian gene expressi...

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Bibliographic Details
Main Authors: Manabu Murakami, Takayoshi Ohba, Agnieszka M Murakami, Chong Han, Kenji Kuwasako, Shirou Itagaki
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0216169

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https://doi.org/10.1371/journal.pone.0216169

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