High-throughput Identification of Phage-derived Imaging Agents

High-throughput Identification of Phage-derived Imaging Agents

The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis me...

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Main Authors: Kimberly A. Kelly, Paul A. Clemons, Amy M. Yu, Ralph Weissleder
Format: Article
Language:English
Published: Hindawi - SAGE Publishing 2006-01-01
Series:Molecular Imaging
Online Access:https://doi.org/10.2310/7290.2006.00003
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spelling doaj-5ff7768cefa444f38d2ae8947658f7922021-04-02T12:43:42ZengHindawi - SAGE PublishingMolecular Imaging1536-01212006-01-01510.2310/7290.2006.0000310.2310_7290.2006.00003High-throughput Identification of Phage-derived Imaging AgentsKimberly A. Kelly0Paul A. Clemons1Amy M. Yu2Ralph Weissleder3Massachusetts General Hospital and Harvard Medical School, USAThe Eli & Edythe Broad Institute of Harvard & MIT, USAMassachusetts General Hospital and Harvard Medical School, USAThe Eli & Edythe Broad Institute of Harvard & MIT, USAThe use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.https://doi.org/10.2310/7290.2006.00003
collection DOAJ
language English
format Article
sources DOAJ
author Kimberly A. Kelly
Paul A. Clemons
Amy M. Yu
Ralph Weissleder
spellingShingle Kimberly A. Kelly
Paul A. Clemons
Amy M. Yu
Ralph Weissleder
High-throughput Identification of Phage-derived Imaging Agents
Molecular Imaging
author_facet Kimberly A. Kelly
Paul A. Clemons
Amy M. Yu
Ralph Weissleder
author_sort Kimberly A. Kelly
title High-throughput Identification of Phage-derived Imaging Agents
title_short High-throughput Identification of Phage-derived Imaging Agents
title_full High-throughput Identification of Phage-derived Imaging Agents
title_fullStr High-throughput Identification of Phage-derived Imaging Agents
title_full_unstemmed High-throughput Identification of Phage-derived Imaging Agents
title_sort high-throughput identification of phage-derived imaging agents
publisher Hindawi - SAGE Publishing
series Molecular Imaging
issn 1536-0121
publishDate 2006-01-01
description The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.
url https://doi.org/10.2310/7290.2006.00003
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AT paulaclemons highthroughputidentificationofphagederivedimagingagents
AT amymyu highthroughputidentificationofphagederivedimagingagents
AT ralphweissleder highthroughputidentificationofphagederivedimagingagents
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