Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique

Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique

A stability-indicating high performance liquid chromatography (HPLC) method is developed for the quantification of efavirenz drug substance. Selected mobile phase is a combination of 50 volume of acetonitrile and 50 volume of 0.86% w/v solution of ammonium dihydrogen phosphate, pH adjusted to 3.0 wi...

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Main Authors: R. Kumar, M. Sharma, G. R. Verma
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:E-Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2011/605381
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spelling doaj-5fecfb9bd7e24c4db32333a8c31c9fef2020-11-24T21:03:59ZengHindawi LimitedE-Journal of Chemistry0973-49452090-98102011-01-01841498150310.1155/2011/605381Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic TechniqueR. Kumar0M. Sharma1G. R. Verma2Department of Chemistry, S. D. (P.G.) College Muzaffarnagar 250001 (U.P.), IndiaDepartment of Pure and Applied Chemistry, M.D.S. University, Ajmer (Rajasthan), IndiaDepartment of Chemistry, S. D. (P.G.) College Muzaffarnagar 250001 (U.P.), IndiaA stability-indicating high performance liquid chromatography (HPLC) method is developed for the quantification of efavirenz drug substance. Selected mobile phase is a combination of 50 volume of acetonitrile and 50 volume of 0.86% w/v solution of ammonium dihydrogen phosphate, pH adjusted to 3.0 with orthophosphoric acid. Optimized column is a stainless steel column packed with base deactivated octa decylsilyl silica gel and at 252 nm wavelength. Linearity of the method is found 40 μg/mL to 160 μg/mL with regression coefficient of 0.9995. The method is validated according to ICH guidelines.http://dx.doi.org/10.1155/2011/605381
collection DOAJ
language English
format Article
sources DOAJ
author R. Kumar
M. Sharma
G. R. Verma
spellingShingle R. Kumar
M. Sharma
G. R. Verma
Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
E-Journal of Chemistry
author_facet R. Kumar
M. Sharma
G. R. Verma
author_sort R. Kumar
title Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
title_short Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
title_full Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
title_fullStr Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
title_full_unstemmed Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
title_sort stability indicating analytical method development and validation of efavirenz quantification by high performance liquid chromatographic technique
publisher Hindawi Limited
series E-Journal of Chemistry
issn 0973-4945
2090-9810
publishDate 2011-01-01
description A stability-indicating high performance liquid chromatography (HPLC) method is developed for the quantification of efavirenz drug substance. Selected mobile phase is a combination of 50 volume of acetonitrile and 50 volume of 0.86% w/v solution of ammonium dihydrogen phosphate, pH adjusted to 3.0 with orthophosphoric acid. Optimized column is a stainless steel column packed with base deactivated octa decylsilyl silica gel and at 252 nm wavelength. Linearity of the method is found 40 μg/mL to 160 μg/mL with regression coefficient of 0.9995. The method is validated according to ICH guidelines.
url http://dx.doi.org/10.1155/2011/605381
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AT msharma stabilityindicatinganalyticalmethoddevelopmentandvalidationofefavirenzquantificationbyhighperformanceliquidchromatographictechnique
AT grverma stabilityindicatinganalyticalmethoddevelopmentandvalidationofefavirenzquantificationbyhighperformanceliquidchromatographictechnique
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