Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain

The search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method. A thermo-halophilic bacterium has been isolated from an undersea hot spring, dubbed Pria Laot Sabang (PLS) A strain. The 0.9 kb DNA Polymerase I...

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Main Authors:	Teuku Mohamad Iqbalsyah, Muhammad A Velayati, Hira Helwati, Febriani Febriani
Format:	Article
Language:	English
Published:	Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University 2020-05-01
Series:	Jurnal Kimia Sains dan Aplikasi
Subjects:	dna polymerase geobacillus halophilic thermophilic sabang
Online Access:	https://ejournal.undip.ac.id/index.php/ksa/article/view/28721

id	doaj-5f92478818db42a4966ce9575dff6489
record_format	Article
spelling	doaj-5f92478818db42a4966ce9575dff64892020-11-25T02:24:41ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142020-05-0123518318810.14710/jksa.23.5.183-18816875Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A StrainTeuku Mohamad Iqbalsyah0Muhammad A Velayati1Hira Helwati2Febriani Febriani3Biomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda AcehBiomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda AcehBiomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda AcehBiomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda AcehThe search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method. A thermo-halophilic bacterium has been isolated from an undersea hot spring, dubbed Pria Laot Sabang (PLS) A strain. The 0.9 kb DNA Polymerase I gene fragments from the isolate were amplified, sequenced, and identified. The fragments were part of the polymerization domain of the enzyme. Homological analysis of the gene sequence showed that the PLS A strain was closely related to Bacillus caldolyticusstrain XM. However, Swissprot structural analysis reveals that PLS A strain had high homology to Geobacillus stearathermophilus. Full sequence analysis is still needed to identify the species and evaluate the intact enzyme structure.https://ejournal.undip.ac.id/index.php/ksa/article/view/28721dna polymerasegeobacillushalophilicthermophilicsabang
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Teuku Mohamad Iqbalsyah Muhammad A Velayati Hira Helwati Febriani Febriani
spellingShingle	Teuku Mohamad Iqbalsyah Muhammad A Velayati Hira Helwati Febriani Febriani Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain Jurnal Kimia Sains dan Aplikasi dna polymerase geobacillus halophilic thermophilic sabang
author_facet	Teuku Mohamad Iqbalsyah Muhammad A Velayati Hira Helwati Febriani Febriani
author_sort	Teuku Mohamad Iqbalsyah
title	Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain
title_short	Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain
title_full	Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain
title_fullStr	Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain
title_full_unstemmed	Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain
title_sort	polymerization domain translated from 0.9 kb gene fragment of dna polymerase i from a thermo-halophilic pls a strain
publisher	Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
series	Jurnal Kimia Sains dan Aplikasi
issn	1410-8917 2597-9914
publishDate	2020-05-01
description	The search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method. A thermo-halophilic bacterium has been isolated from an undersea hot spring, dubbed Pria Laot Sabang (PLS) A strain. The 0.9 kb DNA Polymerase I gene fragments from the isolate were amplified, sequenced, and identified. The fragments were part of the polymerization domain of the enzyme. Homological analysis of the gene sequence showed that the PLS A strain was closely related to Bacillus caldolyticusstrain XM. However, Swissprot structural analysis reveals that PLS A strain had high homology to Geobacillus stearathermophilus. Full sequence analysis is still needed to identify the species and evaluate the intact enzyme structure.
topic	dna polymerase geobacillus halophilic thermophilic sabang
url	https://ejournal.undip.ac.id/index.php/ksa/article/view/28721
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Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain

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