Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors

• Main Authors: Rene Kizek, David Potesil, Vojtech Adam, Jitka Petrlova, Frantisek Jelen, Libuse Trnkova
• Format: Article
• Language: English
• Published: MDPI AG 2005-11-01
• Series: Sensors
• Subjects: Oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides
• Online Access: http://www.mdpi.com/1424-8220/5/6/448/

The paper describes successful coupling of adsorptive transfer stripping (AdTS) andelimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C)and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers)were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing ofthe ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through theelimination function conserving the diffusion current component and eliminating kinetic and chargingcurrent components. This function, which provides the elimination signal in a peak-counterpeak form,increased the current sensitivity for A and C resolution, and for the recognition of bases sequences inODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scanrate were found. The combination of EVLS with AdTS procedure can be considered as a newdetection method in a DNA sensor.