Novel ‘Bacteriospray’ Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity

Novel ‘Bacteriospray’ Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity

Metagenomic techniques, notably the cloning of environmental DNA (eDNA) into surrogate hosts, have given access to the genome of uncultured bacteria. However, the determination of gene functions based on DNA sequences alone remains a significant challenge. The functional screening of metagenomic lib...

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Bibliographic Details
Main Authors: Anissa Brahami, Annie Castonguay, Éric Déziel
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Methods and Protocols
Subjects:
antibiotic
bacterial spray
optimization
library construction
Online Access:http://www.mdpi.com/2409-9279/2/1/4

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