Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay

In our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana (<i>Turnera diffusa</i>) against multiple myeloma (NCI-H929, U266, and MM1S) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an aceton...

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Main Authors:	Johanna Willer, Karin Jöhrer, Richard Greil, Christian Zidorn, Serhat Sezai Çiçek
Format:	Article
Language:	English
Published:	MDPI AG 2019-02-01
Series:	Molecules
Subjects:	multiple myeloma quality control naringenin flavonoids traditional preparation
Online Access:	https://www.mdpi.com/1420-3049/24/5/855

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spelling	doaj-5f530debc9b849358bdc4cb9fad43f5f2020-11-25T02:53:16ZengMDPI AGMolecules1420-30492019-02-0124585510.3390/molecules24050855molecules24050855Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD AssayJohanna Willer0Karin Jöhrer1Richard Greil2Christian Zidorn3Serhat Sezai Çiçek4Department of Pharmaceutical Biology, Kiel University, Gutenbergstraße 76, 24118 Kiel, GermanyTyrolean Cancer Research Institute, Innrain 66, 6020 Innsbruck, AustriaTyrolean Cancer Research Institute, Innrain 66, 6020 Innsbruck, AustriaDepartment of Pharmaceutical Biology, Kiel University, Gutenbergstraße 76, 24118 Kiel, GermanyDepartment of Pharmaceutical Biology, Kiel University, Gutenbergstraße 76, 24118 Kiel, GermanyIn our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana (<i>Turnera diffusa</i>) against multiple myeloma (NCI-H929, U266, and MM1S) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an acetone (70%) crude extract was identified as the most active fraction. Further separation of the active fraction led to the isolation of naringenin (<b>1</b>), three apigenin coumaroyl glucosides <b>2</b>⁻<b>4</b>, and five flavone aglycones <b>5</b>⁻<b>9</b>. Naringenin (<b>1</b>) and apigenin 7-<i>O</i>-(4″-<i>O</i>-<i>p</i>-<i>E</i>-coumaroyl)-glucoside (<b>4</b>) showed significant cytotoxic effects against the tested myeloma cell lines. Additionally, we established a validated ultra-high performance liquid chromatography diode array detector (UHPLC-DAD) method for the quantification of the isolated components in the herb and in traditional preparations of <i>T. diffusa</i>.https://www.mdpi.com/1420-3049/24/5/855multiple myelomaquality controlnaringeninflavonoidstraditional preparation
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Johanna Willer Karin Jöhrer Richard Greil Christian Zidorn Serhat Sezai Çiçek
spellingShingle	Johanna Willer Karin Jöhrer Richard Greil Christian Zidorn Serhat Sezai Çiçek Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay Molecules multiple myeloma quality control naringenin flavonoids traditional preparation
author_facet	Johanna Willer Karin Jöhrer Richard Greil Christian Zidorn Serhat Sezai Çiçek
author_sort	Johanna Willer
title	Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay
title_short	Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay
title_full	Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay
title_fullStr	Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay
title_full_unstemmed	Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay
title_sort	cytotoxic properties of damiana (<i>turnera diffusa</i>) extracts and constituents and a validated quantitative uhplc-dad assay
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2019-02-01
description	In our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana (<i>Turnera diffusa</i>) against multiple myeloma (NCI-H929, U266, and MM1S) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an acetone (70%) crude extract was identified as the most active fraction. Further separation of the active fraction led to the isolation of naringenin (<b>1</b>), three apigenin coumaroyl glucosides <b>2</b>⁻<b>4</b>, and five flavone aglycones <b>5</b>⁻<b>9</b>. Naringenin (<b>1</b>) and apigenin 7-<i>O</i>-(4″-<i>O</i>-<i>p</i>-<i>E</i>-coumaroyl)-glucoside (<b>4</b>) showed significant cytotoxic effects against the tested myeloma cell lines. Additionally, we established a validated ultra-high performance liquid chromatography diode array detector (UHPLC-DAD) method for the quantification of the isolated components in the herb and in traditional preparations of <i>T. diffusa</i>.
topic	multiple myeloma quality control naringenin flavonoids traditional preparation
url	https://www.mdpi.com/1420-3049/24/5/855
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Cytotoxic Properties of Damiana (<i>Turnera diffusa</i>) Extracts and Constituents and A Validated Quantitative UHPLC-DAD Assay

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