| Summary: | In our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana (<i>Turnera diffusa</i>) against multiple myeloma (NCI-H929, U266, and MM1S) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an acetone (70%) crude extract was identified as the most active fraction. Further separation of the active fraction led to the isolation of naringenin (<b>1</b>), three apigenin coumaroyl glucosides <b>2</b>⁻<b>4</b>, and five flavone aglycones <b>5</b>⁻<b>9</b>. Naringenin (<b>1</b>) and apigenin 7-<i>O</i>-(4″-<i>O</i>-<i>p</i>-<i>E</i>-coumaroyl)-glucoside (<b>4</b>) showed significant cytotoxic effects against the tested myeloma cell lines. Additionally, we established a validated ultra-high performance liquid chromatography diode array detector (UHPLC-DAD) method for the quantification of the isolated components in the herb and in traditional preparations of <i>T. diffusa</i>.
|
|---|
