Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
The objective of this work was the study of the relative contribution of general esterases: á-napthyl acetate (á-NA) and p-nitro phenyl acetate (p-NPA) in pyrethroid-resistant T. infestans from these locations. We used deltamethrin-resistant populations previously collected in two areas in Argentina...
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Asociación Toxicológica Argentina
2011-06-01
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doaj-5f30d5c6490a444596349acd1ccbfbfa2020-11-24T23:47:00ZengAsociación Toxicológica ArgentinaActa Toxicológica Argentina0327-92861851-37432011-06-011913240Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and BoliviaPablo Luis Santo-OrihuelaMaría Inés PicolloThe objective of this work was the study of the relative contribution of general esterases: á-napthyl acetate (á-NA) and p-nitro phenyl acetate (p-NPA) in pyrethroid-resistant T. infestans from these locations. We used deltamethrin-resistant populations previously collected in two areas in Argentina (Salta and La Rioja) and one area in Bolivia (Tarija). In this study, frequency histograms of á-NA and p-NPA esterase activities, exhibited similar patterns between Bolivian population and the susceptible counterpart. In contrast, Argentinean field populations showed higher proportion of increased enzyme activity individuals. These results clearly demonstrated that resistances in Argentinean and Bolivian field populations are based on different mechanisms, suggesting that enzyme-based pyrethroid resistance in this species has multiple origins.<br>El objetivo de este trabajo fue el estudio de la contribución relativa de esterasas generales: á-naftil acetato (á-NA) y p-nitro fenil acetato (p-NPA) a la resistencia a piretroides de T. infestans provenientes de esas localidades. Se evaluaron poblaciones resistentes a deltametrina previamente recolectadas en dos áreas de Argentina (Salta y La Rioja) y un área en Bolivia (Tarija). En este estudio, los histogramas de frecuencias de actividades de á-NA y p-NPA esterasas mostraron perfiles similares entre la población boliviana y la población susceptible. Por el contrario, en las poblaciones argentinas de campo se observó una elevada proporción de individuos con actividad enzimática incrementada. Estos resultados demuestran claramente que la resistencia en poblaciones argentinas y bolivianas de campo se origina a partir de distintos mecanismos, sugiriendo que la resistencia a piretroides basada en la acción de enzimas presentan múltiples orígenes en estas especies.http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432011000100004Triatoma infestansResistencia a piretroidesÁ-naftil acetato esterasasP-nitro fenil acetato esterasasTriatoma infestansPyrethroid resistanceÁ-naphtyl acetate esterasesP-nitrophenyl acetate esterases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pablo Luis Santo-Orihuela María Inés Picollo |
spellingShingle |
Pablo Luis Santo-Orihuela María Inés Picollo Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia Acta Toxicológica Argentina Triatoma infestans Resistencia a piretroides Á-naftil acetato esterasas P-nitro fenil acetato esterasas Triatoma infestans Pyrethroid resistance Á-naphtyl acetate esterases P-nitrophenyl acetate esterases |
author_facet |
Pablo Luis Santo-Orihuela María Inés Picollo |
author_sort |
Pablo Luis Santo-Orihuela |
title |
Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia |
title_short |
Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia |
title_full |
Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia |
title_fullStr |
Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia |
title_full_unstemmed |
Contribution of general esterases to pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia |
title_sort |
contribution of general esterases to pyrethroid resistant triatoma infestans (hemiptera: reduviidae) from argentina and bolivia |
publisher |
Asociación Toxicológica Argentina |
series |
Acta Toxicológica Argentina |
issn |
0327-9286 1851-3743 |
publishDate |
2011-06-01 |
description |
The objective of this work was the study of the relative contribution of general esterases: á-napthyl acetate (á-NA) and p-nitro phenyl acetate (p-NPA) in pyrethroid-resistant T. infestans from these locations. We used deltamethrin-resistant populations previously collected in two areas in Argentina (Salta and La Rioja) and one area in Bolivia (Tarija). In this study, frequency histograms of á-NA and p-NPA esterase activities, exhibited similar patterns between Bolivian population and the susceptible counterpart. In contrast, Argentinean field populations showed higher proportion of increased enzyme activity individuals. These results clearly demonstrated that resistances in Argentinean and Bolivian field populations are based on different mechanisms, suggesting that enzyme-based pyrethroid resistance in this species has multiple origins.<br>El objetivo de este trabajo fue el estudio de la contribución relativa de esterasas generales: á-naftil acetato (á-NA) y p-nitro fenil acetato (p-NPA) a la resistencia a piretroides de T. infestans provenientes de esas localidades. Se evaluaron poblaciones resistentes a deltametrina previamente recolectadas en dos áreas de Argentina (Salta y La Rioja) y un área en Bolivia (Tarija). En este estudio, los histogramas de frecuencias de actividades de á-NA y p-NPA esterasas mostraron perfiles similares entre la población boliviana y la población susceptible. Por el contrario, en las poblaciones argentinas de campo se observó una elevada proporción de individuos con actividad enzimática incrementada. Estos resultados demuestran claramente que la resistencia en poblaciones argentinas y bolivianas de campo se origina a partir de distintos mecanismos, sugiriendo que la resistencia a piretroides basada en la acción de enzimas presentan múltiples orígenes en estas especies. |
topic |
Triatoma infestans Resistencia a piretroides Á-naftil acetato esterasas P-nitro fenil acetato esterasas Triatoma infestans Pyrethroid resistance Á-naphtyl acetate esterases P-nitrophenyl acetate esterases |
url |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432011000100004 |
work_keys_str_mv |
AT pabloluissantoorihuela contributionofgeneralesterasestopyrethroidresistanttriatomainfestanshemipterareduviidaefromargentinaandbolivia AT mariainespicollo contributionofgeneralesterasestopyrethroidresistanttriatomainfestanshemipterareduviidaefromargentinaandbolivia |
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