RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II
RNA sequences are expected to be identical to their corresponding DNA sequences. Here, we found all 12 types of RNA-DNA sequence differences (RDDs) in nascent RNA. Our results show that RDDs begin to occur in RNA chains ∼55 nt from the RNA polymerase II (Pol II) active site. These RDDs occur so soon...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2014-03-01
|
Series: | Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714000710 |
id |
doaj-5f2aebfb28ce422f95afdc0f229f309e |
---|---|
record_format |
Article |
spelling |
doaj-5f2aebfb28ce422f95afdc0f229f309e2020-11-25T01:46:35ZengElsevierCell Reports2211-12472014-03-016590691510.1016/j.celrep.2014.01.037RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase IIIsabel X. Wang0Leighton J. Core1Hojoong Kwak2Lauren Brady3Alan Bruzel4Lee McDaniel5Allison L. Richards6Ming Wu7Christopher Grunseich8John T. Lis9Vivian G. Cheung10Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USADepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USACell and Molecular Biology Graduate Program, University of Pennsylvania, Philadelphia, PA 19104, USALife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USADepartment of Genetics, University of Pennsylvania, Philadelphia, PA 19104, USAHuman Genetics Graduate Program, University of Michigan, Ann Arbor, MI 48109, USAHoward Hughes Medical Institute, Chevy Chase, MD 20815, USANeurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USADepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USALife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USARNA sequences are expected to be identical to their corresponding DNA sequences. Here, we found all 12 types of RNA-DNA sequence differences (RDDs) in nascent RNA. Our results show that RDDs begin to occur in RNA chains ∼55 nt from the RNA polymerase II (Pol II) active site. These RDDs occur so soon after transcription that they are incompatible with known deaminase-mediated RNA-editing mechanisms. Moreover, the 55 nt delay in appearance indicates that they do not arise during RNA synthesis by Pol II or as a direct consequence of modified base incorporation. Preliminary data suggest that RDD and R-loop formations may be coupled. These findings identify sequence substitution as an early step in cotranscriptional RNA processing.http://www.sciencedirect.com/science/article/pii/S2211124714000710 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Isabel X. Wang Leighton J. Core Hojoong Kwak Lauren Brady Alan Bruzel Lee McDaniel Allison L. Richards Ming Wu Christopher Grunseich John T. Lis Vivian G. Cheung |
spellingShingle |
Isabel X. Wang Leighton J. Core Hojoong Kwak Lauren Brady Alan Bruzel Lee McDaniel Allison L. Richards Ming Wu Christopher Grunseich John T. Lis Vivian G. Cheung RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II Cell Reports |
author_facet |
Isabel X. Wang Leighton J. Core Hojoong Kwak Lauren Brady Alan Bruzel Lee McDaniel Allison L. Richards Ming Wu Christopher Grunseich John T. Lis Vivian G. Cheung |
author_sort |
Isabel X. Wang |
title |
RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II |
title_short |
RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II |
title_full |
RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II |
title_fullStr |
RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II |
title_full_unstemmed |
RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II |
title_sort |
rna-dna differences are generated in human cells within seconds after rna exits polymerase ii |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2014-03-01 |
description |
RNA sequences are expected to be identical to their corresponding DNA sequences. Here, we found all 12 types of RNA-DNA sequence differences (RDDs) in nascent RNA. Our results show that RDDs begin to occur in RNA chains ∼55 nt from the RNA polymerase II (Pol II) active site. These RDDs occur so soon after transcription that they are incompatible with known deaminase-mediated RNA-editing mechanisms. Moreover, the 55 nt delay in appearance indicates that they do not arise during RNA synthesis by Pol II or as a direct consequence of modified base incorporation. Preliminary data suggest that RDD and R-loop formations may be coupled. These findings identify sequence substitution as an early step in cotranscriptional RNA processing. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124714000710 |
work_keys_str_mv |
AT isabelxwang rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT leightonjcore rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT hojoongkwak rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT laurenbrady rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT alanbruzel rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT leemcdaniel rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT allisonlrichards rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT mingwu rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT christophergrunseich rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT johntlis rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii AT viviangcheung rnadnadifferencesaregeneratedinhumancellswithinsecondsafterrnaexitspolymeraseii |
_version_ |
1725018522934837248 |