Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2

The proliferation and apoptosis of pulmonary artery smooth muscle cells (PASMCs) are considered to be key steps in the progression of pulmonary arterial hypertension (PAH). MicroRNAs (e.g., miR-29b) have been identified in various diseases to be critical modulators of cell growth and apoptosis by ta...

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Main Authors:	Juan Chen, Yanping Li, Yun Li, Lijian Xie, Jianyi Wang, Yongwei Zhang, Tingting Xiao
Format:	Article
Language:	English
Published:	Hindawi Limited 2018-01-01
Series:	BioMed Research International
Online Access:	http://dx.doi.org/10.1155/2018/6051407

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spelling	doaj-5f203a2179cb4c518e60eac14837b6e12020-11-24T22:37:21ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/60514076051407Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2Juan Chen0Yanping Li1Yun Li2Lijian Xie3Jianyi Wang4Yongwei Zhang5Tingting Xiao6Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaDepartment of Cardiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Lu Ding Road, Shanghai 200062, ChinaThe proliferation and apoptosis of pulmonary artery smooth muscle cells (PASMCs) are considered to be key steps in the progression of pulmonary arterial hypertension (PAH). MicroRNAs (e.g., miR-29b) have been identified in various diseases to be critical modulators of cell growth and apoptosis by targeting Mcl-1 and CCND2. However, the role of miR-29b in PAH remains unknown. So we try to investigate the effect of miR-29b on Mcl-1 and CCND2 protein in PASMCs, analyze the effect of miR-29b on the proliferation of PASMCs, and explore the significance of miR-29b in the proliferation, apoptosis, and gene therapy of PAH. It was observed that gene chip analysis showed miR-29b expression in pulmonary artery tissue. The expression of miR-29b was significantly reduced in PAH model mice. MiR-29b inhibited the proliferation of PASMCs and promoted the apoptosis of PASMCs. Mechanically, miR-29b could inhibit the expression of Mcl-1 and CCND2 protein and silenced Mcl-1 and CCND2 could abolish the change of proliferation and apoptosis of PASMCs. These results demonstrate that miR-29b suppressed cellular proliferation and promoted apoptosis of PASMCs, possibly through the inhibition of Mcl-1 and CCND2. Therefore, miR-29b may serve as a useful therapeutic tool to treat PAH.http://dx.doi.org/10.1155/2018/6051407
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Juan Chen Yanping Li Yun Li Lijian Xie Jianyi Wang Yongwei Zhang Tingting Xiao
spellingShingle	Juan Chen Yanping Li Yun Li Lijian Xie Jianyi Wang Yongwei Zhang Tingting Xiao Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2 BioMed Research International
author_facet	Juan Chen Yanping Li Yun Li Lijian Xie Jianyi Wang Yongwei Zhang Tingting Xiao
author_sort	Juan Chen
title	Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2
title_short	Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2
title_full	Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2
title_fullStr	Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2
title_full_unstemmed	Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2
title_sort	effect of mir-29b on the proliferation and apoptosis of pulmonary artery smooth muscle cells by targeting mcl-1 and ccnd2
publisher	Hindawi Limited
series	BioMed Research International
issn	2314-6133 2314-6141
publishDate	2018-01-01
description	The proliferation and apoptosis of pulmonary artery smooth muscle cells (PASMCs) are considered to be key steps in the progression of pulmonary arterial hypertension (PAH). MicroRNAs (e.g., miR-29b) have been identified in various diseases to be critical modulators of cell growth and apoptosis by targeting Mcl-1 and CCND2. However, the role of miR-29b in PAH remains unknown. So we try to investigate the effect of miR-29b on Mcl-1 and CCND2 protein in PASMCs, analyze the effect of miR-29b on the proliferation of PASMCs, and explore the significance of miR-29b in the proliferation, apoptosis, and gene therapy of PAH. It was observed that gene chip analysis showed miR-29b expression in pulmonary artery tissue. The expression of miR-29b was significantly reduced in PAH model mice. MiR-29b inhibited the proliferation of PASMCs and promoted the apoptosis of PASMCs. Mechanically, miR-29b could inhibit the expression of Mcl-1 and CCND2 protein and silenced Mcl-1 and CCND2 could abolish the change of proliferation and apoptosis of PASMCs. These results demonstrate that miR-29b suppressed cellular proliferation and promoted apoptosis of PASMCs, possibly through the inhibition of Mcl-1 and CCND2. Therefore, miR-29b may serve as a useful therapeutic tool to treat PAH.
url	http://dx.doi.org/10.1155/2018/6051407
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Effect of miR-29b on the Proliferation and Apoptosis of Pulmonary Artery Smooth Muscle Cells by Targeting Mcl-1 and CCND2

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