Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)

Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morpholo...

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Main Authors: Manjunath M. Hulikere, Chandrashekhar G. Joshi, D. Ananda, Jagadeesh Poyya, T. Nivya
Format: Article
Language:English
Published: Taylor & Francis Group 2016-10-01
Series:Mycology
Subjects:
Online Access:http://dx.doi.org/10.1080/21501203.2016.1263688
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spelling doaj-5f1266de9cf8438ab12e5b0b8f9e39542020-11-24T23:15:28ZengTaylor & Francis GroupMycology2150-12032150-12112016-10-017420321110.1080/21501203.2016.12636881263688Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)Manjunath M. Hulikere0Chandrashekhar G. Joshi1D. Ananda2Jagadeesh Poyya3T. Nivya4Mangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraEndophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morphological characters and the rDNA internal transcribed spacer sequence analysis (BLAST search in Gen Bank database) was used for the identification of endophytic fungus. The antioxidant potential of the ethyl acetate extract of endophytic fungus was assessed by, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging method. The fungal extract was also analysed for reducing power, total phenolic and flavonoid content. Antiangiogenic activity of the fungal extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by Chick chorioallantoic membrane assay. The endophytic fungus was identified as Cladosporium cladosporioides (Gen Bank ID – KT384175). The ethyl acetate extract of C. cladosporioides showed a significant antioxidant and angiosuppressive activity. The ESI-LC-MS analysis of the extract revealed the presence of wide range of secondary metabolites. Results suggest that C. cladosporioides extract could be exploited as a potential source for angiogenic modulators.http://dx.doi.org/10.1080/21501203.2016.1263688Cladosporium cladosporioidesSargassum wightiiangiogenesisantioxidant activityPolyphenolsCAM assay
collection DOAJ
language English
format Article
sources DOAJ
author Manjunath M. Hulikere
Chandrashekhar G. Joshi
D. Ananda
Jagadeesh Poyya
T. Nivya
spellingShingle Manjunath M. Hulikere
Chandrashekhar G. Joshi
D. Ananda
Jagadeesh Poyya
T. Nivya
Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
Mycology
Cladosporium cladosporioides
Sargassum wightii
angiogenesis
antioxidant activity
Polyphenols
CAM assay
author_facet Manjunath M. Hulikere
Chandrashekhar G. Joshi
D. Ananda
Jagadeesh Poyya
T. Nivya
author_sort Manjunath M. Hulikere
title Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
title_short Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
title_full Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
title_fullStr Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
title_full_unstemmed Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
title_sort antiangiogenic, wound healing and antioxidant activity of cladosporium cladosporioides (endophytic fungus) isolated from seaweed (sargassum wightii)
publisher Taylor & Francis Group
series Mycology
issn 2150-1203
2150-1211
publishDate 2016-10-01
description Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morphological characters and the rDNA internal transcribed spacer sequence analysis (BLAST search in Gen Bank database) was used for the identification of endophytic fungus. The antioxidant potential of the ethyl acetate extract of endophytic fungus was assessed by, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging method. The fungal extract was also analysed for reducing power, total phenolic and flavonoid content. Antiangiogenic activity of the fungal extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by Chick chorioallantoic membrane assay. The endophytic fungus was identified as Cladosporium cladosporioides (Gen Bank ID – KT384175). The ethyl acetate extract of C. cladosporioides showed a significant antioxidant and angiosuppressive activity. The ESI-LC-MS analysis of the extract revealed the presence of wide range of secondary metabolites. Results suggest that C. cladosporioides extract could be exploited as a potential source for angiogenic modulators.
topic Cladosporium cladosporioides
Sargassum wightii
angiogenesis
antioxidant activity
Polyphenols
CAM assay
url http://dx.doi.org/10.1080/21501203.2016.1263688
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