Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)
Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morpholo...
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doaj-5f1266de9cf8438ab12e5b0b8f9e39542020-11-24T23:15:28ZengTaylor & Francis GroupMycology2150-12032150-12112016-10-017420321110.1080/21501203.2016.12636881263688Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii)Manjunath M. Hulikere0Chandrashekhar G. Joshi1D. Ananda2Jagadeesh Poyya3T. Nivya4Mangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraMangalore University, P.G.Centre, Chikka AluvaraEndophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morphological characters and the rDNA internal transcribed spacer sequence analysis (BLAST search in Gen Bank database) was used for the identification of endophytic fungus. The antioxidant potential of the ethyl acetate extract of endophytic fungus was assessed by, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging method. The fungal extract was also analysed for reducing power, total phenolic and flavonoid content. Antiangiogenic activity of the fungal extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by Chick chorioallantoic membrane assay. The endophytic fungus was identified as Cladosporium cladosporioides (Gen Bank ID – KT384175). The ethyl acetate extract of C. cladosporioides showed a significant antioxidant and angiosuppressive activity. The ESI-LC-MS analysis of the extract revealed the presence of wide range of secondary metabolites. Results suggest that C. cladosporioides extract could be exploited as a potential source for angiogenic modulators.http://dx.doi.org/10.1080/21501203.2016.1263688Cladosporium cladosporioidesSargassum wightiiangiogenesisantioxidant activityPolyphenolsCAM assay |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manjunath M. Hulikere Chandrashekhar G. Joshi D. Ananda Jagadeesh Poyya T. Nivya |
spellingShingle |
Manjunath M. Hulikere Chandrashekhar G. Joshi D. Ananda Jagadeesh Poyya T. Nivya Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii) Mycology Cladosporium cladosporioides Sargassum wightii angiogenesis antioxidant activity Polyphenols CAM assay |
author_facet |
Manjunath M. Hulikere Chandrashekhar G. Joshi D. Ananda Jagadeesh Poyya T. Nivya |
author_sort |
Manjunath M. Hulikere |
title |
Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii) |
title_short |
Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii) |
title_full |
Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii) |
title_fullStr |
Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii) |
title_full_unstemmed |
Antiangiogenic, wound healing and antioxidant activity of Cladosporium cladosporioides (Endophytic Fungus) isolated from seaweed (Sargassum wightii) |
title_sort |
antiangiogenic, wound healing and antioxidant activity of cladosporium cladosporioides (endophytic fungus) isolated from seaweed (sargassum wightii) |
publisher |
Taylor & Francis Group |
series |
Mycology |
issn |
2150-1203 2150-1211 |
publishDate |
2016-10-01 |
description |
Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morphological characters and the rDNA internal transcribed spacer sequence analysis (BLAST search in Gen Bank database) was used for the identification of endophytic fungus. The antioxidant potential of the ethyl acetate extract of endophytic fungus was assessed by, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging method. The fungal extract was also analysed for reducing power, total phenolic and flavonoid content. Antiangiogenic activity of the fungal extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by Chick chorioallantoic membrane assay. The endophytic fungus was identified as Cladosporium cladosporioides (Gen Bank ID – KT384175). The ethyl acetate extract of C. cladosporioides showed a significant antioxidant and angiosuppressive activity. The ESI-LC-MS analysis of the extract revealed the presence of wide range of secondary metabolites. Results suggest that C. cladosporioides extract could be exploited as a potential source for angiogenic modulators. |
topic |
Cladosporium cladosporioides Sargassum wightii angiogenesis antioxidant activity Polyphenols CAM assay |
url |
http://dx.doi.org/10.1080/21501203.2016.1263688 |
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