Mortality in juvenile stages of the Andean Spiny Toad Rhinella spinulosa (Wiegman 1843): an indicator of waters contaminated by anti-inflammatory drugs

• Main Authors: Rayme-Chalco Marisol, Tenorio-Bautista Saturnino Martín
• Format: Article
• Language: Spanish
• Published: Selva Andina Research Society 2020-08-01
• Series: Journal of the Selva Andina Research Society
• Subjects: accumulated mortality; waters contaminated by drugs; immature stages of rhinella spinulosa; lc50.
• Online Access: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2072-92942020000200007&lng=es&nrm=iso&tlng=en
• ISSN: 2072-9294; 2072-9308

This research has been developed in the Biodiversity and Geographic Information System Laboratory at the Faculty of Biological Sciences of National University of San Cristobal de Huamanga. Forty experimental units of 1L round plastic containers were built with different concentrations of anti-inflammatory drugs and control units. Ten immature specimens of Rhinella spinulosa (Wiegman 1843) about 2 to 25 cm were placed in each experimental unit to determine mortality rates during continuous exposure at 24 and 48 h. As a result, mortality percentages obtained were statistically different (p <0.05); ibuprofen has generated a mortality of 43.33 and 100% at 40 and 80 mg/L of concentration for 24 hours and 40, 76.67 and 100% a 10; 20 and 40 mg/L of concentration for 48 hours, naproxen 3.33 and 13.33% at 32 and 64 mg/L of concentration for 24 hours and 23.33; 26.67; 50 and 73.33% at 8, 16; 32 y 64 mg/L of concentration for 48 hours, and diclofenac 13.33 y 100% at 60 y 120 mg/L for 24 hours and 23.33; 60, 90 y 100% at 15; 30; 60 y 120 mg/L for 48 hours. The LC50 was respectively: 68.6 mg / L, 106.6 mg / L and 120 mg / L for 24 hours and 13.47 mg / L, 104.6 mg / L and 29.8 mg / L for 48 hours, showing that as the exposure time decreases the lethal concentration increase.