PREPARATION OF ZINC ENRICHED YEAST (SACCHAROMYCES CEREVISIAE) BY CULTIVATION WITH DIFFERENT ZINC SALTS

The yeast Saccharomyces cerevisiae is the best known microorganism and therefore widely used in many branches of industry. This study aims to investigate the accumulation of three inorganic zinc salts. Our research presents the ability of this yeast to absorb zinc from liquid medium and such enriche...

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Bibliographic Details
Main Authors: Ľuboš Harangozo, Alena Vollmannová, Anežka Poláková, Dana Urminská, Blažena Lavová, Silvia Šillerová
Format: Article
Language:English
Published: Slovak University of Agriculture 2012-02-01
Series:Journal of Microbiology, Biotechnology and Food Sciences
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Online Access:http://www.jmbfs.org/wp-content/uploads/2012/01/Sillerova1.pdf
Description
Summary:The yeast Saccharomyces cerevisiae is the best known microorganism and therefore widely used in many branches of industry. This study aims to investigate the accumulation of three inorganic zinc salts. Our research presents the ability of this yeast to absorb zinc from liquid medium and such enriched biomass use as a potential source of microelements in animal and/or human nutrition. It was found that the addition of different zinc forms, i.e. zinc nitrate, zinc sulphate and zinc chloride in fixed concentrations of 0, 25, 50 and 100 mg.100 ml-1 did not affect the amount of dry yeast biomass yielded, i.e. 1.0 – 1.2 g of yeast cells from 100 ml of cultivation medium, while higher presence of zinc solutions caused significantly lower yield of yeast biomass. The highest amount of zinc in yeast cells was achieved when added in the form of zinc nitrate in concentration of 200 mg.100 ml-1 YPD medium. The increment of intracellular zinc was up to 18.5 mg.g-1 of yeast biomass.
ISSN:1338-5178