Single-Cell Tracking of Breast Cancer Cells Enables Prediction of Sphere Formation from Early Cell Divisions

Summary: The mammosphere assay has become widely employed to quantify stem-like cells in a population. However, the problem is there is no standard protocol employed by the field. Cell seeding densities of 1,000 to 100,000 cells/mL have been reported. These high densities lead to cellular aggregatio...

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Bibliographic Details
Main Authors: Patrick C. Bailey, Rachel M. Lee, Michele I. Vitolo, Stephen J.P. Pratt, Eleanor Ory, Kristi Chakrabarti, Cornell J. Lee, Keyata N. Thompson, Stuart S. Martin
Format: Article
Language:English
Published: Elsevier 2018-10-01
Series:iScience
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004218301275