Description of Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae) isolated from hilly areas of Kashmir Valley

• Main Authors: Aashaq Hussain Bhat, Tarique Hassan Askary, Mohammad Jamal Ahmad, Suman, Aasha, Ashok Kumar Chaubey
• Format: Article
• Language: English
• Published: SpringerOpen 2019-12-01
• Series: Egyptian Journal of Biological Pest Control
• Subjects: Entomopathogenic nematodes; Heterorhabditis; ITS; Meta-analysis; Distribution
• Online Access: https://doi.org/10.1186/s41938-019-0197-6

Abstract Three strains of entomopathogenic nematodes, labelled P5, P6 and PH, were isolated during surveys of agricultural soils of Pir Panjal Range, using insect baiting technique. Morpho-taxometrical studies and molecular data confirmed that these isolates belong to Heterorhabditis bacteriophora, making this finding the first report of this species from Jammu and Kashmir, India. Their distribution using a meta-analysis of GenBank records was attempted to assess. The morphology, morphometric studies and molecular data were conspecific to original description with minor deviations. Data analysis of the distribution showed that H. bacteriophora was the most ubiquitous throughout the South Africa subcontinent, but it was rarely found in Indian subcontinent having been isolated from 3 states throughout the country. As these 3 strains of H. bacteriophora are native to the hilly region of Kashmir Valley, they can be exploited for the control of target crop insect pests of the region. However, further studies are required regarding their life cycle, host range, virulence potential and survival capacity under extreme environmental conditions.