Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, w...
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doaj-5e7ad28e50b94b88bb348f8300d1c24a2020-11-25T02:17:28ZengPortland Press, Biochemical SocietyBioscience Reports1573-49352014-03-01342e0010110.1042/BSR20130095BSR20130095Establishment and biological characterization of a dermal mesenchymal stem cells line from bovineTingting SunChao Yu0Yuhua Gao1Chenqiong Zhao2Jinlian Hua3Lianshun Cai4Weijun Guan5Yuehui Ma6 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Harbin Institute Of Physical Education, Harbin 150000, People's Republic of China College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory for Animal Biotechnology of Agriculture Ministry of China, Northwest A & F University, Yangling, Shanxi 712100, People's Republic of China Institute of Basic Medicine, Jiamusi University, Jiamusi 154007, People's Republic of China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.http://www.bioscirep.org/bsr/034/e101/bsr034e101.htmbovinecell culturedermal mesenchymal stem cell (DMSC)multi-lineage differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tingting Sun Chao Yu Yuhua Gao Chenqiong Zhao Jinlian Hua Lianshun Cai Weijun Guan Yuehui Ma |
spellingShingle |
Tingting Sun Chao Yu Yuhua Gao Chenqiong Zhao Jinlian Hua Lianshun Cai Weijun Guan Yuehui Ma Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine Bioscience Reports bovine cell culture dermal mesenchymal stem cell (DMSC) multi-lineage differentiation |
author_facet |
Tingting Sun Chao Yu Yuhua Gao Chenqiong Zhao Jinlian Hua Lianshun Cai Weijun Guan Yuehui Ma |
author_sort |
Tingting Sun |
title |
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_short |
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_full |
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_fullStr |
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_full_unstemmed |
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_sort |
establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
publisher |
Portland Press, Biochemical Society |
series |
Bioscience Reports |
issn |
1573-4935 |
publishDate |
2014-03-01 |
description |
The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells. |
topic |
bovine cell culture dermal mesenchymal stem cell (DMSC) multi-lineage differentiation |
url |
http://www.bioscirep.org/bsr/034/e101/bsr034e101.htm |
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