Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine

The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, w...

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Main Authors: Tingting Sun, Chao Yu, Yuhua Gao, Chenqiong Zhao, Jinlian Hua, Lianshun Cai, Weijun Guan, Yuehui Ma
Format: Article
Language:English
Published: Portland Press, Biochemical Society 2014-03-01
Series:Bioscience Reports
Subjects:
Online Access:http://www.bioscirep.org/bsr/034/e101/bsr034e101.htm
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spelling doaj-5e7ad28e50b94b88bb348f8300d1c24a2020-11-25T02:17:28ZengPortland Press, Biochemical SocietyBioscience Reports1573-49352014-03-01342e0010110.1042/BSR20130095BSR20130095Establishment and biological characterization of a dermal mesenchymal stem cells line from bovineTingting SunChao Yu0Yuhua Gao1Chenqiong Zhao2Jinlian Hua3Lianshun Cai4Weijun Guan5Yuehui Ma6 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Harbin Institute Of Physical Education, Harbin 150000, People's Republic of China College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Laboratory for Animal Biotechnology of Agriculture Ministry of China, Northwest A & F University, Yangling, Shanxi 712100, People's Republic of China Institute of Basic Medicine, Jiamusi University, Jiamusi 154007, People's Republic of China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.http://www.bioscirep.org/bsr/034/e101/bsr034e101.htmbovinecell culturedermal mesenchymal stem cell (DMSC)multi-lineage differentiation
collection DOAJ
language English
format Article
sources DOAJ
author Tingting Sun
Chao Yu
Yuhua Gao
Chenqiong Zhao
Jinlian Hua
Lianshun Cai
Weijun Guan
Yuehui Ma
spellingShingle Tingting Sun
Chao Yu
Yuhua Gao
Chenqiong Zhao
Jinlian Hua
Lianshun Cai
Weijun Guan
Yuehui Ma
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
Bioscience Reports
bovine
cell culture
dermal mesenchymal stem cell (DMSC)
multi-lineage differentiation
author_facet Tingting Sun
Chao Yu
Yuhua Gao
Chenqiong Zhao
Jinlian Hua
Lianshun Cai
Weijun Guan
Yuehui Ma
author_sort Tingting Sun
title Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_short Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_full Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_fullStr Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_full_unstemmed Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_sort establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
publisher Portland Press, Biochemical Society
series Bioscience Reports
issn 1573-4935
publishDate 2014-03-01
description The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.
topic bovine
cell culture
dermal mesenchymal stem cell (DMSC)
multi-lineage differentiation
url http://www.bioscirep.org/bsr/034/e101/bsr034e101.htm
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