CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure

CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure

The role of muscle LIM protein (MLP), encoded by CSRP3, is not well understood in humans. CSRP3 knockout mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Using CRISPR/Cas9, we generated an MLP deficient human ESC line WAe009-A-41 carrying a compound heterozygous...

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Bibliographic Details
Main Authors:	Liqiang Sun, Jing Li, En Li, Shaohui Niu, Zhiping Qin, Qing Zhi, Jiajia Zhao, Haiyan Xiong, Yuan Li, Liguo Jian, Lihua Zhang
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506120303780

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