CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure

The role of muscle LIM protein (MLP), encoded by CSRP3, is not well understood in humans. CSRP3 knockout mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Using CRISPR/Cas9, we generated an MLP deficient human ESC line WAe009-A-41 carrying a compound heterozygous...

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Main Authors:	Liqiang Sun, Jing Li, En Li, Shaohui Niu, Zhiping Qin, Qing Zhi, Jiajia Zhao, Haiyan Xiong, Yuan Li, Liguo Jian, Lihua Zhang
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506120303780

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spelling	doaj-5e6f6bb0384140cda652642aa348052a2020-12-19T05:05:04ZengElsevierStem Cell Research1873-50612020-12-0149102077CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failureLiqiang Sun0Jing Li1En Li2Shaohui Niu3Zhiping Qin4Qing Zhi5Jiajia Zhao6Haiyan Xiong7Yuan Li8Liguo Jian9Lihua Zhang10Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Nuclear Medicine, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Doppler Ultrasonic, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Corresponding authors at: The Second Affiliated Hospital of Zhengzhou University, Zhengzhou University, Jingba Road No. 2, Jinshui District, Zhengzhou 450052, China.Department of Cardiology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Corresponding authors at: The Second Affiliated Hospital of Zhengzhou University, Zhengzhou University, Jingba Road No. 2, Jinshui District, Zhengzhou 450052, China.The role of muscle LIM protein (MLP), encoded by CSRP3, is not well understood in humans. CSRP3 knockout mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Using CRISPR/Cas9, we generated an MLP deficient human ESC line WAe009-A-41 carrying a compound heterozygous 13 bp deletion/1 bp insertion in the CSRP3 gene. The WAe009-A-41 line exhibited a normal female karyotype (46, XX), expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. MLP expression was not detectable in WAe009-A-41 line. This cell line can be a useful tool for studying the role of CSRP3 in cardiomyopathy and heart failure.http://www.sciencedirect.com/science/article/pii/S1873506120303780
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Liqiang Sun Jing Li En Li Shaohui Niu Zhiping Qin Qing Zhi Jiajia Zhao Haiyan Xiong Yuan Li Liguo Jian Lihua Zhang
spellingShingle	Liqiang Sun Jing Li En Li Shaohui Niu Zhiping Qin Qing Zhi Jiajia Zhao Haiyan Xiong Yuan Li Liguo Jian Lihua Zhang CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure Stem Cell Research
author_facet	Liqiang Sun Jing Li En Li Shaohui Niu Zhiping Qin Qing Zhi Jiajia Zhao Haiyan Xiong Yuan Li Liguo Jian Lihua Zhang
author_sort	Liqiang Sun
title	CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure
title_short	CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure
title_full	CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure
title_fullStr	CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure
title_full_unstemmed	CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure
title_sort	crispr/cas9 mediated establishment of a human csrp3 compound heterozygous knockout hesc line to model cardiomyopathy and heart failure
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2020-12-01
description	The role of muscle LIM protein (MLP), encoded by CSRP3, is not well understood in humans. CSRP3 knockout mice developed dilated cardiomyopathy with hypertrophy and heart failure after birth. Using CRISPR/Cas9, we generated an MLP deficient human ESC line WAe009-A-41 carrying a compound heterozygous 13 bp deletion/1 bp insertion in the CSRP3 gene. The WAe009-A-41 line exhibited a normal female karyotype (46, XX), expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. MLP expression was not detectable in WAe009-A-41 line. This cell line can be a useful tool for studying the role of CSRP3 in cardiomyopathy and heart failure.
url	http://www.sciencedirect.com/science/article/pii/S1873506120303780
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CRISPR/Cas9 mediated establishment of a human CSRP3 compound heterozygous knockout hESC line to model cardiomyopathy and heart failure

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