Separation of mono- and diglycerides by gas-liquid chromatography
The parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for trigl...
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1969-01-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752042646X |
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doaj-5e23fede5e384da58deec3f627de24432021-04-24T05:54:21ZengElsevierJournal of Lipid Research0022-22751969-01-011013340Separation of mono- and diglycerides by gas-liquid chromatographyRodney Watts0R. Dils1Department of Biochemistry, University of Birmingham, Birmingham, EnglandDepartment of Biochemistry, University of Birmingham, Birmingham, EnglandThe parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for triglycerides.The isothermal characteristics of a range of trimethylsilyl ethers of mono- and diglycerides on both stationary phases showed that log retention volume was directly proportional to carbon number and inversely proportional to absolute temperature. However, glyceride derivatives with lower carbon numbers deviated from these relationships.By using various rates of programmed temperature rise, we have determined the elution temperatures (Kelvin scale) of the mono- and diglyceride trimethylsilyl ethers relative to that of glycerol trilaurate. The “carbon equivalent of a trimethylsilyl group” is defined and shown to be useful in comparing the chromatographic properties of different glyceride classes.Weight and molar correction factors have been obtained and used to analyze diglycerides derived from egg and bovine brain lecithins.http://www.sciencedirect.com/science/article/pii/S002222752042646Xtrimethylsilylationisothermatemperature programmingelution temperaturescorrection factors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rodney Watts R. Dils |
spellingShingle |
Rodney Watts R. Dils Separation of mono- and diglycerides by gas-liquid chromatography Journal of Lipid Research trimethylsilylation isotherma temperature programming elution temperatures correction factors |
author_facet |
Rodney Watts R. Dils |
author_sort |
Rodney Watts |
title |
Separation of mono- and diglycerides by gas-liquid chromatography |
title_short |
Separation of mono- and diglycerides by gas-liquid chromatography |
title_full |
Separation of mono- and diglycerides by gas-liquid chromatography |
title_fullStr |
Separation of mono- and diglycerides by gas-liquid chromatography |
title_full_unstemmed |
Separation of mono- and diglycerides by gas-liquid chromatography |
title_sort |
separation of mono- and diglycerides by gas-liquid chromatography |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1969-01-01 |
description |
The parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for triglycerides.The isothermal characteristics of a range of trimethylsilyl ethers of mono- and diglycerides on both stationary phases showed that log retention volume was directly proportional to carbon number and inversely proportional to absolute temperature. However, glyceride derivatives with lower carbon numbers deviated from these relationships.By using various rates of programmed temperature rise, we have determined the elution temperatures (Kelvin scale) of the mono- and diglyceride trimethylsilyl ethers relative to that of glycerol trilaurate. The “carbon equivalent of a trimethylsilyl group” is defined and shown to be useful in comparing the chromatographic properties of different glyceride classes.Weight and molar correction factors have been obtained and used to analyze diglycerides derived from egg and bovine brain lecithins. |
topic |
trimethylsilylation isotherma temperature programming elution temperatures correction factors |
url |
http://www.sciencedirect.com/science/article/pii/S002222752042646X |
work_keys_str_mv |
AT rodneywatts separationofmonoanddiglyceridesbygasliquidchromatography AT rdils separationofmonoanddiglyceridesbygasliquidchromatography |
_version_ |
1721511391447220224 |