Separation of mono- and diglycerides by gas-liquid chromatography

The parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for trigl...

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Main Authors: Rodney Watts, R. Dils
Format: Article
Language:English
Published: Elsevier 1969-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752042646X
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spelling doaj-5e23fede5e384da58deec3f627de24432021-04-24T05:54:21ZengElsevierJournal of Lipid Research0022-22751969-01-011013340Separation of mono- and diglycerides by gas-liquid chromatographyRodney Watts0R. Dils1Department of Biochemistry, University of Birmingham, Birmingham, EnglandDepartment of Biochemistry, University of Birmingham, Birmingham, EnglandThe parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for triglycerides.The isothermal characteristics of a range of trimethylsilyl ethers of mono- and diglycerides on both stationary phases showed that log retention volume was directly proportional to carbon number and inversely proportional to absolute temperature. However, glyceride derivatives with lower carbon numbers deviated from these relationships.By using various rates of programmed temperature rise, we have determined the elution temperatures (Kelvin scale) of the mono- and diglyceride trimethylsilyl ethers relative to that of glycerol trilaurate. The “carbon equivalent of a trimethylsilyl group” is defined and shown to be useful in comparing the chromatographic properties of different glyceride classes.Weight and molar correction factors have been obtained and used to analyze diglycerides derived from egg and bovine brain lecithins.http://www.sciencedirect.com/science/article/pii/S002222752042646Xtrimethylsilylationisothermatemperature programmingelution temperaturescorrection factors
collection DOAJ
language English
format Article
sources DOAJ
author Rodney Watts
R. Dils
spellingShingle Rodney Watts
R. Dils
Separation of mono- and diglycerides by gas-liquid chromatography
Journal of Lipid Research
trimethylsilylation
isotherma
temperature programming
elution temperatures
correction factors
author_facet Rodney Watts
R. Dils
author_sort Rodney Watts
title Separation of mono- and diglycerides by gas-liquid chromatography
title_short Separation of mono- and diglycerides by gas-liquid chromatography
title_full Separation of mono- and diglycerides by gas-liquid chromatography
title_fullStr Separation of mono- and diglycerides by gas-liquid chromatography
title_full_unstemmed Separation of mono- and diglycerides by gas-liquid chromatography
title_sort separation of mono- and diglycerides by gas-liquid chromatography
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1969-01-01
description The parameters affecting the separation and quantification of trimethylsilyl ethers of mono- and diglycerides have been investigated by gas-liquid chromatography with QF-1 and SE-30 as stationary phases and a flame ionization detector. Results have been compared with those obtained earlier for triglycerides.The isothermal characteristics of a range of trimethylsilyl ethers of mono- and diglycerides on both stationary phases showed that log retention volume was directly proportional to carbon number and inversely proportional to absolute temperature. However, glyceride derivatives with lower carbon numbers deviated from these relationships.By using various rates of programmed temperature rise, we have determined the elution temperatures (Kelvin scale) of the mono- and diglyceride trimethylsilyl ethers relative to that of glycerol trilaurate. The “carbon equivalent of a trimethylsilyl group” is defined and shown to be useful in comparing the chromatographic properties of different glyceride classes.Weight and molar correction factors have been obtained and used to analyze diglycerides derived from egg and bovine brain lecithins.
topic trimethylsilylation
isotherma
temperature programming
elution temperatures
correction factors
url http://www.sciencedirect.com/science/article/pii/S002222752042646X
work_keys_str_mv AT rodneywatts separationofmonoanddiglyceridesbygasliquidchromatography
AT rdils separationofmonoanddiglyceridesbygasliquidchromatography
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