Therapeutic effect of aqueous extract of Globularia alypum (Ga) following insulin toxicity induced in vitro on cardiomyocytes Rattus norvegicus

• Main Authors: SMAIL LEILA, BERDJA SIHEM, SAKA BOUALEM, NEGGAZI SAMIA, BOUMAZA SALIHA, KACIMI GHOUTI, BOUDARENE LYNDA, AOUICHAT BOUGUERRA SOUHILA
• Format: Article
• Language: English
• Published: Phytochemistry & Organic Synthesis Laboratory 2017-11-01
• Series: PhytoChem & BioSub Journal
• Online Access: https://drive.google.com/file/d/19abDcz4s1GKm7hQcegNfg-c4cvw6H-jt/view?usp=sharing
• ISSN: 2170-1768; 2170-1768

Introduction: Insulin resistance marked by an inflammatory response via production TNF α and compensatory hyperinsulinemia leading to long term installation of glucolipotoxicity and type 2 diabetes. Material and Methods : In this study, we analyzed the effect of polyphenol exracts Globularia alypum (Ga) in vitro, following an induced stress on the cardiomyocyte of Rattus norvegicus by the addition of a high dose of insulin (10 UI/ml). In this case, we evaluated the proliferation rate by counting and evaluated the cellular content on nitric oxide (NO), malondialdehyde (MDA) and catalase. Results and Discussion: In the presence of high dose of insulin, cardiomyocytes showed deceased cell survival, an imbalance of redox status in favor of increase of NO and MDA and decrease of an enzyme of the antioxidant system, catalase. Following the action of Ga polyphenol extracts, we recorded an improvement in the rate of cell proliferation, decreased levels of NO and MDA and increased levels of catalase activity. Conclusion: the state of stress induced insulin resistance mimed by the high dose of insulin of cardiomyocytes marked by physiological and biochemical alterations. These, apoptosis precursors are attenuated after addition of polyphenol exracts Globularia alypum which may be a therapeutic target upstream on the installation of T2DM.