Kesesuaian Hasil Pemeriksaan Antibodi Virus Herpes Simpleks Metode Enzyme-Linked Immunofiltration Assay dengan Enzyme-Linked Immunosorbent Assay

• Main Authors: Victor Immanuel, Noormartany, Nina Susana Dewi
• Format: Article
• Language: English
• Published: Universitas Padjajaran 2012-09-01
• Series: Majalah Kedokteran Bandung
• Subjects: ELISA method; ELIFA method; anti-HSV IgM
• Online Access: http://journal.fk.unpad.ac.id/index.php/mkb/article/view/86

Herpes simplex virus (HSV) infections are very common and are caused by HSV type 1 (HSV-1) and HSV type 2 (HSV-2). HSV-1 being mostly associated with orofacial disease, whereas HSV-2 is usually associated with perigenital infection. Diagnosis of HSV infection is established based on history, physical and laboratory examination. Enzyme-linked immunosorbent assay (ELISA) method to detect anti-HSV has a sensitivity 93–100% and specificity 95–100%, whereas enzyme-linked immunofiltration assay (ELIFA) has a sensitivity 83.36–97% and specificity 83.93–98%. The aim of this study was to assess the agreement of anti-HSV between ELIFA and ELISA methods. This study was conducted in the clinical laboratory RSUP Dr. Hasan Sadikin Bandung since January to May 2011. The study design was cross sectional. Subjects of this study were serum of patients suspected HSV infection. Statistical analysis was performed to assess Kappa agreement. A total of 66 samples were examined anti-HSV using ELIFA and ELISA method. There was good agreement between test results of anti-HSV IgM ELIFA and ELISA method (p<0.001, κ=0.621), moderate agreement between test results of anti- HSV-1 IgG ELIFA and ELISA method (p<0.001, κ=0.533), and fair agreement between test results of anti-HSV-2 IgG ELIFA and ELISA method (p=0.006, κ= 0.260). In conclusions, only the anti-HSV IgM ELIFA method has good agreement with ELISA.