NADH-GOGAT Overexpression Does Not Improve Maize (<i>Zea mays</i> L.) Performance Even When Pyramiding with NAD-IDH, GDH and GS

• Main Authors: Rafael A. Cañas, Zhazira Yesbergenova-Cuny, Léo Belanger, Jacques Rouster, Lenaïg Brulé, Françoise Gilard, Isabelle Quilleré, Christophe Sallaud, Bertrand Hirel
• Format: Article
• Language: English
• Published: MDPI AG 2020-01-01
• Series: Plants
• Subjects: amino acids; biomass; carbon; genetic manipulation; kernel yield; maize; nitrogen; organic acids
• Online Access: https://www.mdpi.com/2223-7747/9/2/130
• ISSN: 2223-7747

Maize plants overexpressing NADH-GOGAT were produced in order to determine if boosting 2-Oxoglurate production used as a carbon skeleton for the biosynthesis of amino acids will improve plant biomass and kernel production. The NADH-GOGAT enzyme recycles glutamate and incorporates carbon skeletons into the ammonium assimilation pathway using the organic acid 2-Oxoglutarate as a substrate. Gene pyramiding was then conducted with NAD-IDH and NADH-GDH, two enzymes also involved in the synthesis of 2-Oxoglurate. NADH-GOGAT overexpression was detrimental for shoot biomass production but did not markedly affect kernel yield. Additional NAD-IDH and NADH-GDH activity did not improve plant performance. A decrease in kernel production was observed when NADH-GDH was pyramided to NADH-GOGAT and NAD-IDH. This decrease could not be restored even when additional cytosolic GS activity was present in the plants overexpressing the three enzymes producing 2-Oxoglutarate. Detailed leaf metabolic profiling of the different transgenic plants revealed that the NADH-GOGAT over-expressors were characterized by an accumulation of amino acids derived from glutamate and a decrease in the amount of carbohydrates further used to provide carbon skeletons for its synthesis. The study suggests that 2-Oxoglutarate synthesis is a key element acting at the interface of carbohydrate and amino acid metabolism and that its accumulation induces an imbalance of primary carbon and nitrogen metabolism that is detrimental for maize productivity.