Characterisation of Biologically Active Hydrolysates and Peptide Fractions of Vacuum Packaging String Bean (<i>Phaseolus vulgaris </i>L.)

• Main Authors: Anna Jakubczyk, Monika Karaś, Piotr Stanikowski, Beata Rutkowska, Magdalena Dziedzic, Ewelina Zielińska, Konrad A. Szychowski, Urszula E. Binduga, Kamila Rybczyńska-Tkaczyk, Barbara Baraniak
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: Foods
• Subjects: bioactive peptides; string bean; nutritional potential; vacuum-packing; in vitro digestion
• Online Access: https://www.mdpi.com/2304-8158/9/7/842
• ISSN: 2304-8158

The aim of the study is to characterise biologically active hydolysates and peptide fractions obtained from vacuum-packed string beans (<i>Phaseolus vulragis</i> L.) (PB). Unpacked beans were a control sample. The influence on human squamous carcinoma cell line SCC-15 (ATCC CRL-1623) was determined. Packed bean (PB) and unpacked bean (UB) extracts were found to exert no effect on the tongue squamous carcinoma cells. The results of the study indicated that the packing process contributed to the retention of protein, soluble dietary fibre, and free sugar (2.36, 3.5, and 1.79 g/100 d.m., respectively). PB was characterised by higher antioxidant activity (expressed as neutralisation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS ABTS^•⁺) and 2,2-diphenyl-1-picrylhydrazyl (DPPH^·) free radicals) as well as Fe²⁺ chelation and reducing power (IC₅₀ = 54.56, 0.46, 3.85 mg mL⁻¹; 0.088 A_{700/peptide content}, respectively) than the UB samples before hydrolysis. The hydrolysis process enhanced these properties. The IC₅₀ value of lipase and α-amylase inhibitory activity of the hydrolysates obtained from UB was reduced. The PB and UB fractions exhibited a certain level of antimicrobial activity against <i>S. aureus </i>and <i>E. coli</i>. <i>Candida albicans</i> were not sensitive to these peptide fractions.